caa a22 4500 605501246 CHVBK 20210128100559.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00253-015-6682-2 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6682-2 Enhanced rifamycin SV production by submerged fermentation using Amycolatopsis mediterranei [Elektronische Daten] [M. Nagavalli, S. Ponamgi, V. Girijashankar, L. Rao] Rifamycin is a broad-spectrum antimicrobial drug produced commercially by submerged fermentation where the yields are far less in comparison to its demand in human drug therapy. Addressing the need, sequential mutational strain improvement was carried using UV and EtBr that resulted in improved strain yielding rifamycin SV up to 4.32g/L. Further optimization of six important fermentation factors was followed which include temperature, agitation, inoculum level, period of fermentation, inorganic nitrogen source and amino acids. For the first time, we report a maximum yield of 5.32g/L of rifamycin SV. Among the amino acids, proline known for its slowest assimilation by Amycolatopsis mediterranei produced the highest improvement in antibiotic yields. Following mutational strain improvement and process optimization, a total of 3.8-fold increase in antibiotic titre was achieved. Following a conventional procedure of mutational strain improvement, highest yield of rifamycin SV was reported by optimizing submerged fermentation process. Springer-Verlag Berlin Heidelberg, 2015 Rifamycin SV nationallicence Amycolatopsis mediterranei nationallicence Mutational strain improvement nationallicence Submerged fermentation optimization nationallicence Proline nationallicence Nagavalli M. Department of Microbiology, Osmania University, 500 007, Hyderabad, India aut Ponamgi S. Center for Biotechnology, Jawaharlal Nehru Technological University, IST, Kukatpally, Hyderabad, India aut Girijashankar V. Scientist C, Department of Science and Technology, DST, New Mehrauli, New Delhi, India aut Rao L. Department of Microbiology, Osmania University, 500 007, Hyderabad, India aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/18(2015-09-01), 7505-7513 0175-7598 99:18<7505 2015 99 253 https://doi.org/10.1007/s00253-015-6682-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6682-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nagavalli M. Department of Microbiology, Osmania University, 500 007, Hyderabad, India aut NATIONALLICENCE 700 1- Ponamgi S. Center for Biotechnology, Jawaharlal Nehru Technological University, IST, Kukatpally, Hyderabad, India aut NATIONALLICENCE 700 1- Girijashankar V. Scientist C, Department of Science and Technology, DST, New Mehrauli, New Delhi, India aut NATIONALLICENCE 700 1- Rao L. Department of Microbiology, Osmania University, 500 007, Hyderabad, India aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/18(2015-09-01), 7505-7513 0175-7598 99:18<7505 2015 99 253