caa a22 4500 605501254 CHVBK 20210128100600.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00253-015-6633-y doi (NATIONALLICENCE)springer-10.1007/s00253-015-6633-y Bacterial community diversity in municipal waste landfill sites [Elektronische Daten] [Liyan Song, Yangqing Wang, Wei Tang, Yu Lei] Little is known about the bacterial diversity of landfills and how environmental factors impact the diversity. In this study, PCR-based 454 pyrosequencing was used to investigate the bacterial communities of ten landfill leachate samples from five landfill sites in China. A total of 137K useable sequences from the V3-V6 regions of the 16S rRNA gene were retrieved from 205K reads. These sequences revealed the presence of a large number of operational taxonomic units (OTUs) in the landfills (709-1599 OTUs per sample). The most predominant bacterial representatives in the landfills investigated, regardless of geographic area, included Gammaproteobacteria, Firmicutes, and Bacteroidetes. The phyla Fusobacteria and Tenericutes were also found for the first time to be predominant in the landfills. The phylum Fusobacteria predominated (51.5 and 48.8%) in two semi-arid landfills, and the phylum Tenericutes dominated (30.6%) at one humid, subtropical landfill. Further, a large number of Pseudomonas was detected in most samples, comprising the dominant group and accounting for 40.9 to 92.4% of the total abundance. Principal component analysis (PCA) and cluster analysis based on OTU abundance showed that the abundant taxa separated the bacterial community. Canonical correlation analysis (CCA) suggested that precipitation and landfilling age significantly impact on the bacterial community structure. The bacterial community function (e.g., cellulolytic bacteria, sulfate-reducing bacteria (SRB), sulfate-oxidizing bacteria, and xenobiotic organic compound (XOC)-degrading bacteria) was also diverse, but the pattern is unclear. Springer-Verlag Berlin Heidelberg, 2015 Landfill nationallicence Landfill leachate nationallicence Bacterial community diversity nationallicence Fusobacteria and Tenericutes nationallicence Pseudomonas nationallicence Song Liyan Research Center of Environmental Microbiology and Ecology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, No 266 Fangzhen Avenue, Shuitu High-Tech Park, Beibei, 400714, Chongqing, China aut Wang Yangqing Research Center of Environmental Microbiology and Ecology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, No 266 Fangzhen Avenue, Shuitu High-Tech Park, Beibei, 400714, Chongqing, China aut Tang Wei Research Center of Environmental Microbiology and Ecology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, No 266 Fangzhen Avenue, Shuitu High-Tech Park, Beibei, 400714, Chongqing, China aut Lei Yu Research Center of Environmental Microbiology and Ecology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, No 266 Fangzhen Avenue, Shuitu High-Tech Park, Beibei, 400714, Chongqing, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/18(2015-09-01), 7745-7756 0175-7598 99:18<7745 2015 99 253 https://doi.org/10.1007/s00253-015-6633-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6633-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Song Liyan Research Center of Environmental Microbiology and Ecology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, No 266 Fangzhen Avenue, Shuitu High-Tech Park, Beibei, 400714, Chongqing, China aut NATIONALLICENCE 700 1- Wang Yangqing Research Center of Environmental Microbiology and Ecology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, No 266 Fangzhen Avenue, Shuitu High-Tech Park, Beibei, 400714, Chongqing, China aut NATIONALLICENCE 700 1- Tang Wei Research Center of Environmental Microbiology and Ecology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, No 266 Fangzhen Avenue, Shuitu High-Tech Park, Beibei, 400714, Chongqing, China aut NATIONALLICENCE 700 1- Lei Yu Research Center of Environmental Microbiology and Ecology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, No 266 Fangzhen Avenue, Shuitu High-Tech Park, Beibei, 400714, Chongqing, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/18(2015-09-01), 7745-7756 0175-7598 99:18<7745 2015 99 253