Metabolic engineering of Escherichia coli for production of biodiesel from fatty alcohols and acetyl-CoA

Verfasser / Beitragende:
[Daoyi Guo, Hong Pan, Xun Li]
Ort, Verlag, Jahr:
2015
Enthalten in:
Applied Microbiology and Biotechnology, 99/18(2015-09-01), 7805-7812
Format:
Artikel (online)
ID: 605501262
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024 7 0 |a 10.1007/s00253-015-6809-5  |2 doi 
035 |a (NATIONALLICENCE)springer-10.1007/s00253-015-6809-5 
245 0 0 |a Metabolic engineering of Escherichia coli for production of biodiesel from fatty alcohols and acetyl-CoA  |h [Elektronische Daten]  |c [Daoyi Guo, Hong Pan, Xun Li] 
520 3 |a Microbial production of biodiesel from renewable feedstock has attracted intensive attention. Biodiesel is known to be produced from short-chain alcohols and fatty acyl-CoAs through the expression of wax ester synthase/fatty acyl-CoA: diacylglycerol acyltransferase that catalyzes the esterification of short-chain alcohols and fatty acyl-CoAs. Here, we engineered Escherichia coli to produce various fatty alcohol acetate esters, which depend on the expression of Saccharomyces cerevisiae alcohol acetyltransferase ATF1 that catalyzes the esterification of fatty alcohols and acetyl-CoA. The fatty acid biosynthetic pathways generate fatty acyl-ACPs, fatty acyl-CoAs, or fatty acids, which can be converted to fatty alcohols by fatty acyl-CoA reductase, fatty acyl-ACP reductase, or carboxylic acid reductase, respectively. This study showed the biosynthesis of biodiesel from three fatty acid biosynthetic pathway intermediates. 
540 |a Springer-Verlag Berlin Heidelberg, 2015 
690 7 |a Biodiesel  |2 nationallicence 
690 7 |a Fatty acids  |2 nationallicence 
690 7 |a Fatty alcohols  |2 nationallicence 
690 7 |a Acetyl-CoA  |2 nationallicence 
700 1 |a Guo  |D Daoyi  |u College of Life and Environmental Sciences, Gannan Normal University, Ganzhou, People's Republic of China  |4 aut 
700 1 |a Pan  |D Hong  |u Key Laboratory of Organo-Pharmaceutical Chemistry, Gannan Normal University, 341000, Ganzhou, Jiangxi Province, People's Republic of China  |4 aut 
700 1 |a Li  |D Xun  |u Key Laboratory of Organo-Pharmaceutical Chemistry, Gannan Normal University, 341000, Ganzhou, Jiangxi Province, People's Republic of China  |4 aut 
773 0 |t Applied Microbiology and Biotechnology  |d Springer Berlin Heidelberg  |g 99/18(2015-09-01), 7805-7812  |x 0175-7598  |q 99:18<7805  |1 2015  |2 99  |o 253 
856 4 0 |u https://doi.org/10.1007/s00253-015-6809-5  |q text/html  |z Onlinezugriff via DOI 
898 |a BK010053  |b XK010053  |c XK010000 
900 7 |a Metadata rights reserved  |b Springer special CC-BY-NC licence  |2 nationallicence 
908 |D 1  |a research-article  |2 jats 
949 |B NATIONALLICENCE  |F NATIONALLICENCE  |b NL-springer 
950 |B NATIONALLICENCE  |P 856  |E 40  |u https://doi.org/10.1007/s00253-015-6809-5  |q text/html  |z Onlinezugriff via DOI 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Guo  |D Daoyi  |u College of Life and Environmental Sciences, Gannan Normal University, Ganzhou, People's Republic of China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Pan  |D Hong  |u Key Laboratory of Organo-Pharmaceutical Chemistry, Gannan Normal University, 341000, Ganzhou, Jiangxi Province, People's Republic of China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Li  |D Xun  |u Key Laboratory of Organo-Pharmaceutical Chemistry, Gannan Normal University, 341000, Ganzhou, Jiangxi Province, People's Republic of China  |4 aut 
950 |B NATIONALLICENCE  |P 773  |E 0-  |t Applied Microbiology and Biotechnology  |d Springer Berlin Heidelberg  |g 99/18(2015-09-01), 7805-7812  |x 0175-7598  |q 99:18<7805  |1 2015  |2 99  |o 253