caa a22 4500 605501335 CHVBK 20210128100600.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00253-015-6661-7 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6661-7 Distribution and population structure characteristics of microorganisms in urban sewage system [Elektronische Daten] [Yanchen Liu, Qian Dong, Hanchang Shi] The sewage system functions as an important public infrastructure. The survived microbial population inside the sewage system plays an important role in the biochemical process during wastewater transportation within the system. The study aims to investigate the microbial communities spatial distribution inside manholes and sewage pipes by using the massive parallel 454 pyrosequencing combined with denaturing gradient gel electrophoresis of V1-V3 regions of 16S rRNA. The microbial structure, distribution characteristic, taxonomic composition analysis, and compositional overlaps of the microbial community both were conducted. The result indicated that the changes in microbial diversity exhibited a consistent trend with average dehydrogenase activity. Proteobacteria, Firmicutes, and Anaerolineae were the dominant bacteria in the sewage system. The microbial community exhibited distinguishing characteristics in comparison with fecal, surface water, and wastewater treatment process. Parachlamydia acanthamoebae, Zymophilus paucivorans, and uncultured Epsilon proteobacterium were mainly found at the upper position of the manhole, while Microbacterium sp. was mainly found at the lower position. Longilinea, Georgenia, and Desulforhabdus were mainly observed in the sewage pipe. The microbial bacteria that survived in the anaerobic environment (i.e., sulfate reduction bacteria groups) exhibited a significant positive relationship with anaerobic crucial environmental factors in the redundancy analysis. Springer-Verlag Berlin Heidelberg, 2015 Sewage system nationallicence Microbial diversity nationallicence Manhole nationallicence Sewage pipe nationallicence Sediment nationallicence Population structure nationallicence Liu Yanchen State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control, Tsinghua University, 100084, Beijing, China aut Dong Qian State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control, Tsinghua University, 100084, Beijing, China aut Shi Hanchang State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control, Tsinghua University, 100084, Beijing, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/18(2015-09-01), 7723-7734 0175-7598 99:18<7723 2015 99 253 https://doi.org/10.1007/s00253-015-6661-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6661-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Yanchen State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Dong Qian State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Shi Hanchang State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/18(2015-09-01), 7723-7734 0175-7598 99:18<7723 2015 99 253