caa a22 4500 605501343 CHVBK 20210128100600.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00253-015-6787-7 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6787-7 Prediction of T cell epitopes of Brucella abortus and evaluation of their protective role in mice [Elektronische Daten] [Prachiti Afley, Sudhir Dohre, G. Prasad, Subodh Kumar] Brucellae are Gram-negative intracellular bacteria that cause an important zoonotic disease called brucellosis. The animal vaccines are available but have disadvantage of causing abortions in a proportion of pregnant animals. The animal vaccines are also pathogenic to humans. Recent trend in vaccine design has shifted to epitope-based vaccines that are safe and specific. In this study, efforts were made to identify MHC-I- and MHC-II-restricted T cell epitopes of Brucella abortus and evaluate their vaccine potential in mice. The peptides were designed using online available immunoinformatics tools, and five MHC-I- and one MHC-II-restricted T cell peptides were selected on the basis of their ability to produce interferon gamma (IFN-γ) in in vivo studies. The selected peptides were co-administered with poly dl-lactide-co-glycolide (PLG) microparticles and evaluated for immunogenicity and protection in BALB/c mice. Mice immunized with peptides either entrapped in PLG microparticles (EPLG-Pep) or adsorbed on PLG particles (APLG-Pep) showed significantly higher splenocyte proliferation and IFN-γ generation to all selected peptides than the mice immunized with corresponding irrelevant peptides formulated PLG microparticles or phosphate-buffered saline (PBS). A significant protection compared to PBS control was also observed in EPLG-Pep and APLG-Pep groups. A plasmid DNA vaccine construct (pVaxPep) for peptides encoding DNA sequences was generated and injected to mice by in vivo electroporation. Significant protection was observed (1.66 protection units) when compared with PBS and empty vector control group animals. Overall, the MHC-I and MHC-II peptides identified in this study are immunogenic and protective in mouse model and support the feasibility of peptide-based vaccine for brucellosis. Springer-Verlag Berlin Heidelberg, 2015 Brucella abortus nationallicence Epitope nationallicence DNA vaccine nationallicence Immunoinformatics nationallicence PLG microparticles nationallicence Afley Prachiti Defence Research & Development Establishment, Jhansi Road, 474002, Gwalior, Madhya Pradesh, India aut Dohre Sudhir Defence Research & Development Establishment, Jhansi Road, 474002, Gwalior, Madhya Pradesh, India aut Prasad G. School of Studies in Biochemistry, Jiwaji University, 474002, Gwalior, Madhya Pradesh, India aut Kumar Subodh Defence Research & Development Establishment, Jhansi Road, 474002, Gwalior, Madhya Pradesh, India aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/18(2015-09-01), 7625-7637 0175-7598 99:18<7625 2015 99 253 https://doi.org/10.1007/s00253-015-6787-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6787-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Afley Prachiti Defence Research & Development Establishment, Jhansi Road, 474002, Gwalior, Madhya Pradesh, India aut NATIONALLICENCE 700 1- Dohre Sudhir Defence Research & Development Establishment, Jhansi Road, 474002, Gwalior, Madhya Pradesh, India aut NATIONALLICENCE 700 1- Prasad G. School of Studies in Biochemistry, Jiwaji University, 474002, Gwalior, Madhya Pradesh, India aut NATIONALLICENCE 700 1- Kumar Subodh Defence Research & Development Establishment, Jhansi Road, 474002, Gwalior, Madhya Pradesh, India aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/18(2015-09-01), 7625-7637 0175-7598 99:18<7625 2015 99 253