Separation and purification of the antioxidant compound hispidin from mushrooms by molecularly imprinted polymer

Verfasser / Beitragende:
[Ning Li, Lijie Zhao, Tzi Ng, Jack Wong, Yan Yan, Zhong Shi, Fang Liu]
Ort, Verlag, Jahr:
2015
Enthalten in:
Applied Microbiology and Biotechnology, 99/18(2015-09-01), 7569-7577
Format:
Artikel (online)
ID: 605501378
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024 7 0 |a 10.1007/s00253-015-6499-z  |2 doi 
035 |a (NATIONALLICENCE)springer-10.1007/s00253-015-6499-z 
245 0 0 |a Separation and purification of the antioxidant compound hispidin from mushrooms by molecularly imprinted polymer  |h [Elektronische Daten]  |c [Ning Li, Lijie Zhao, Tzi Ng, Jack Wong, Yan Yan, Zhong Shi, Fang Liu] 
520 3 |a A new molecularly imprinted polymer (MIP), prepared by hispidin as the template molecule, was synthesized and applied as an adsorbent phase for solid phase extraction (SPE) to isolate and enrich hispidin from eight species of mushrooms. The optimization of synthesis and the adsorption behaviors of the MIPs were investigated in detail. In comparison with C18-SPE, MIP-SPE displayed high selectivity and good affinity for hispidin for extract of Phellinus igniarius. The antioxidant activity of the extracts after using the MIPs was evaluated by free radical scavenging activity, and inhibition of erythrocyte hemolysis, and lipid peroxidation. This developed method provided a rapid, selective, and effective approach for separation and enrichment of active compounds from the natural products. 
540 |a Springer-Verlag Berlin Heidelberg, 2015 
690 7 |a Hispidin  |2 nationallicence 
690 7 |a Molecularly imprinted polymer  |2 nationallicence 
690 7 |a Mushrooms  |2 nationallicence 
690 7 |a Antioxidant activity  |2 nationallicence 
700 1 |a Li  |D Ning  |u Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China  |4 aut 
700 1 |a Zhao  |D Lijie  |u Key Laboratory of Original Environmental Quality of MOA, Agro-Environmental Protection Institute of Ministry of Agriculture, 300191, Tianjin, People's Republic of China  |4 aut 
700 1 |a Ng  |D Tzi  |u School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, New Territories, China  |4 aut 
700 1 |a Wong  |D Jack  |u School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, New Territories, China  |4 aut 
700 1 |a Yan  |D Yan  |u Department of Microbiology, College of Life Science, Nankai University, 300071, Tianjin, China  |4 aut 
700 1 |a Shi  |D Zhong  |u Department of Microbiology, College of Life Science, Nankai University, 300071, Tianjin, China  |4 aut 
700 1 |a Liu  |D Fang  |u Department of Microbiology, College of Life Science, Nankai University, 300071, Tianjin, China  |4 aut 
773 0 |t Applied Microbiology and Biotechnology  |d Springer Berlin Heidelberg  |g 99/18(2015-09-01), 7569-7577  |x 0175-7598  |q 99:18<7569  |1 2015  |2 99  |o 253 
856 4 0 |u https://doi.org/10.1007/s00253-015-6499-z  |q text/html  |z Onlinezugriff via DOI 
898 |a BK010053  |b XK010053  |c XK010000 
900 7 |a Metadata rights reserved  |b Springer special CC-BY-NC licence  |2 nationallicence 
908 |D 1  |a research-article  |2 jats 
949 |B NATIONALLICENCE  |F NATIONALLICENCE  |b NL-springer 
950 |B NATIONALLICENCE  |P 856  |E 40  |u https://doi.org/10.1007/s00253-015-6499-z  |q text/html  |z Onlinezugriff via DOI 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Li  |D Ning  |u Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Zhao  |D Lijie  |u Key Laboratory of Original Environmental Quality of MOA, Agro-Environmental Protection Institute of Ministry of Agriculture, 300191, Tianjin, People's Republic of China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Ng  |D Tzi  |u School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, New Territories, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Wong  |D Jack  |u School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, New Territories, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Yan  |D Yan  |u Department of Microbiology, College of Life Science, Nankai University, 300071, Tianjin, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Shi  |D Zhong  |u Department of Microbiology, College of Life Science, Nankai University, 300071, Tianjin, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Liu  |D Fang  |u Department of Microbiology, College of Life Science, Nankai University, 300071, Tianjin, China  |4 aut 
950 |B NATIONALLICENCE  |P 773  |E 0-  |t Applied Microbiology and Biotechnology  |d Springer Berlin Heidelberg  |g 99/18(2015-09-01), 7569-7577  |x 0175-7598  |q 99:18<7569  |1 2015  |2 99  |o 253