caa a22 4500 605501378 CHVBK 20210128100600.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00253-015-6499-z doi (NATIONALLICENCE)springer-10.1007/s00253-015-6499-z Separation and purification of the antioxidant compound hispidin from mushrooms by molecularly imprinted polymer [Elektronische Daten] [Ning Li, Lijie Zhao, Tzi Ng, Jack Wong, Yan Yan, Zhong Shi, Fang Liu] A new molecularly imprinted polymer (MIP), prepared by hispidin as the template molecule, was synthesized and applied as an adsorbent phase for solid phase extraction (SPE) to isolate and enrich hispidin from eight species of mushrooms. The optimization of synthesis and the adsorption behaviors of the MIPs were investigated in detail. In comparison with C18-SPE, MIP-SPE displayed high selectivity and good affinity for hispidin for extract of Phellinus igniarius. The antioxidant activity of the extracts after using the MIPs was evaluated by free radical scavenging activity, and inhibition of erythrocyte hemolysis, and lipid peroxidation. This developed method provided a rapid, selective, and effective approach for separation and enrichment of active compounds from the natural products. Springer-Verlag Berlin Heidelberg, 2015 Hispidin nationallicence Molecularly imprinted polymer nationallicence Mushrooms nationallicence Antioxidant activity nationallicence Li Ning Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China aut Zhao Lijie Key Laboratory of Original Environmental Quality of MOA, Agro-Environmental Protection Institute of Ministry of Agriculture, 300191, Tianjin, People's Republic of China aut Ng Tzi School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, New Territories, China aut Wong Jack School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, New Territories, China aut Yan Yan Department of Microbiology, College of Life Science, Nankai University, 300071, Tianjin, China aut Shi Zhong Department of Microbiology, College of Life Science, Nankai University, 300071, Tianjin, China aut Liu Fang Department of Microbiology, College of Life Science, Nankai University, 300071, Tianjin, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/18(2015-09-01), 7569-7577 0175-7598 99:18<7569 2015 99 253 https://doi.org/10.1007/s00253-015-6499-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6499-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Ning Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China aut NATIONALLICENCE 700 1- Zhao Lijie Key Laboratory of Original Environmental Quality of MOA, Agro-Environmental Protection Institute of Ministry of Agriculture, 300191, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Ng Tzi School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, New Territories, China aut NATIONALLICENCE 700 1- Wong Jack School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, New Territories, China aut NATIONALLICENCE 700 1- Yan Yan Department of Microbiology, College of Life Science, Nankai University, 300071, Tianjin, China aut NATIONALLICENCE 700 1- Shi Zhong Department of Microbiology, College of Life Science, Nankai University, 300071, Tianjin, China aut NATIONALLICENCE 700 1- Liu Fang Department of Microbiology, College of Life Science, Nankai University, 300071, Tianjin, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/18(2015-09-01), 7569-7577 0175-7598 99:18<7569 2015 99 253