Acetoclastic methanogenesis is likely the dominant biochemical pathway of palmitate degradation in the presence of sulfate
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 605501416 | ||
| 003 | CHVBK | ||
| 005 | 20210128100600.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 210128e20150901xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6669-z |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6669-z | ||
| 245 | 0 | 0 | |a Acetoclastic methanogenesis is likely the dominant biochemical pathway of palmitate degradation in the presence of sulfate |h [Elektronische Daten] |c [Lei Lv, Serge Mbadinga, Li-Ying Wang, Jin-Feng Liu, Ji-Dong Gu, Bo-Zhong Mu, Shi-Zhong Yang] |
| 520 | 3 | |a Long chain fatty acids (LCFAs) are important intermediates in the anaerobic degradation of n-alkanes. In order to find out the biochemical processes involved in the degradation of LCFAs, palmitate (a typical LCFA) was used as a substrate, and low-temperature oilfield production fluids were used as a source of microorganisms to establish two anaerobic systems, one with addition of sulfate as exogenous electron acceptor (SP), another without exogenous electron acceptor (MP) and both incubated at room temperature. After more than 2years of incubation, about 48 and 57.4% of the palmitate were degraded in samples of MP and SP, respectively. Methane production reached 1408 and 1064μmol for MP and SP, respectively. Clone libraries of archaeal 16S rRNA genes showed that the predominant archaea in the sulfate-amended cultures (SP) was Methanosaeta whereas Methanocalculus dominated the culture without addition of exogenous sulfate (MP). This observation shows that palmitate could be biodegraded into methane through β-oxidation and acetoclastic methanogenesis in the presence of with or without sulfate. The high occurrence of Methanosaeta in the sulfate-amended system indicates that acetoclastic methanogenesis was not inhibited/little affected by the addition of sulfate. Acetoclastic methanogenesis might be the predominant biochemchimcal pathway of methane generation in enrichment cultures amended with sulfate. These results shed light on alternative methanogenic pathways in the presence of sulfate. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Anaerobic degradation |2 nationallicence | |
| 690 | 7 | |a Hydrogenotrophic |2 nationallicence | |
| 690 | 7 | |a Acetoclastic |2 nationallicence | |
| 690 | 7 | |a Methanogenesis |2 nationallicence | |
| 690 | 7 | |a Palmitate |2 nationallicence | |
| 690 | 7 | |a Sulfate reduction |2 nationallicence | |
| 700 | 1 | |a Lv |D Lei |u State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China |4 aut | |
| 700 | 1 | |a Mbadinga |D Serge |u State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China |4 aut | |
| 700 | 1 | |a Wang |D Li-Ying |u State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China |4 aut | |
| 700 | 1 | |a Liu |D Jin-Feng |u State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China |4 aut | |
| 700 | 1 | |a Gu |D Ji-Dong |u School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, SAR, People's Republic of China |4 aut | |
| 700 | 1 | |a Mu |D Bo-Zhong |u State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China |4 aut | |
| 700 | 1 | |a Yang |D Shi-Zhong |u State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/18(2015-09-01), 7757-7769 |x 0175-7598 |q 99:18<7757 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6669-z |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6669-z |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lv |D Lei |u State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mbadinga |D Serge |u State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wang |D Li-Ying |u State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Jin-Feng |u State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gu |D Ji-Dong |u School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, SAR, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mu |D Bo-Zhong |u State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yang |D Shi-Zhong |u State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/18(2015-09-01), 7757-7769 |x 0175-7598 |q 99:18<7757 |1 2015 |2 99 |o 253 | ||