caa a22 4500 605501416 CHVBK 20210128100600.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00253-015-6669-z doi (NATIONALLICENCE)springer-10.1007/s00253-015-6669-z Acetoclastic methanogenesis is likely the dominant biochemical pathway of palmitate degradation in the presence of sulfate [Elektronische Daten] [Lei Lv, Serge Mbadinga, Li-Ying Wang, Jin-Feng Liu, Ji-Dong Gu, Bo-Zhong Mu, Shi-Zhong Yang] Long chain fatty acids (LCFAs) are important intermediates in the anaerobic degradation of n-alkanes. In order to find out the biochemical processes involved in the degradation of LCFAs, palmitate (a typical LCFA) was used as a substrate, and low-temperature oilfield production fluids were used as a source of microorganisms to establish two anaerobic systems, one with addition of sulfate as exogenous electron acceptor (SP), another without exogenous electron acceptor (MP) and both incubated at room temperature. After more than 2years of incubation, about 48 and 57.4% of the palmitate were degraded in samples of MP and SP, respectively. Methane production reached 1408 and 1064μmol for MP and SP, respectively. Clone libraries of archaeal 16S rRNA genes showed that the predominant archaea in the sulfate-amended cultures (SP) was Methanosaeta whereas Methanocalculus dominated the culture without addition of exogenous sulfate (MP). This observation shows that palmitate could be biodegraded into methane through β-oxidation and acetoclastic methanogenesis in the presence of with or without sulfate. The high occurrence of Methanosaeta in the sulfate-amended system indicates that acetoclastic methanogenesis was not inhibited/little affected by the addition of sulfate. Acetoclastic methanogenesis might be the predominant biochemchimcal pathway of methane generation in enrichment cultures amended with sulfate. These results shed light on alternative methanogenic pathways in the presence of sulfate. Springer-Verlag Berlin Heidelberg, 2015 Anaerobic degradation nationallicence Hydrogenotrophic nationallicence Acetoclastic nationallicence Methanogenesis nationallicence Palmitate nationallicence Sulfate reduction nationallicence Lv Lei State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut Mbadinga Serge State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut Wang Li-Ying State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut Liu Jin-Feng State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut Gu Ji-Dong School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, SAR, People's Republic of China aut Mu Bo-Zhong State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut Yang Shi-Zhong State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/18(2015-09-01), 7757-7769 0175-7598 99:18<7757 2015 99 253 https://doi.org/10.1007/s00253-015-6669-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6669-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lv Lei State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Mbadinga Serge State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Wang Li-Ying State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Liu Jin-Feng State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Gu Ji-Dong School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, SAR, People's Republic of China aut NATIONALLICENCE 700 1- Mu Bo-Zhong State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Yang Shi-Zhong State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/18(2015-09-01), 7757-7769 0175-7598 99:18<7757 2015 99 253