caa a22 4500 605501424 CHVBK 20210128100600.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00253-015-6474-8 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6474-8 Characterisation of a novel thermostable endoglucanase from Alicyclobacillus vulcanalis of potential application in bioethanol production [Elektronische Daten] [Angela Boyce, Gary Walsh] A novel endoglucanase encoding gene was cloned from Alicyclobacillus vulcanalis and expressed in E. coli. The deduced amino acid sequence showed highest identity with α-l-arabinofuranosidase-like proteins from glycoside hydrolase family 51. The recombinant enzyme was purified by affinity chromatography and characterised in terms of its potential suitability for lignocellulose hydrolysis at high temperature in the production of bioethanol. The purified enzyme displayed maximum activity at 80°C and pH 3.6-4.5. Tween 20 was found to have a beneficial effect on enzyme activity and thermal stability. When incubated in the presence of 0.1% Tween 20, the enzyme retained full activity after 72h at 70°C and 78% of original activity after 72h at 75°C. Maximum activity was observed on carboxymethyl cellulose, and the purified enzyme also hydrolysed lichenan, barley β-glucan and xylan. The purified enzyme decreased the viscosity of carboxymethyl cellulose when assessed at 70-85°C and was capable of releasing reducing sugars from acid-pretreated straw at 70 and 75°C. The results indicate the potential suitability of the enzyme for industrial application in the production of cellulosic bioethanol. Springer-Verlag Berlin Heidelberg, 2015 Endoglucanase nationallicence Alicyclobacillus vulcanalis nationallicence Thermostable nationallicence Lignocellulose nationallicence Boyce Angela Department of Chemical and Environmental Sciences and Materials and Surface Science Institute, University of Limerick, Limerick, Ireland aut Walsh Gary Department of Chemical and Environmental Sciences and Materials and Surface Science Institute, University of Limerick, Limerick, Ireland aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/18(2015-09-01), 7515-7525 0175-7598 99:18<7515 2015 99 253 https://doi.org/10.1007/s00253-015-6474-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6474-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Boyce Angela Department of Chemical and Environmental Sciences and Materials and Surface Science Institute, University of Limerick, Limerick, Ireland aut NATIONALLICENCE 700 1- Walsh Gary Department of Chemical and Environmental Sciences and Materials and Surface Science Institute, University of Limerick, Limerick, Ireland aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/18(2015-09-01), 7515-7525 0175-7598 99:18<7515 2015 99 253