caa a22 4500 605501475 CHVBK 20210128100601.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00253-015-6795-7 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6795-7 Proteome signatures—how are they obtained and what do they teach us? [Elektronische Daten] [João da Costa, Virginia Carvalhais, Rita Ferreira, Francisco Amado, Manuel Vilanova, Nuno Cerca, Rui Vitorino] The dawn of a new Proteomics era, just over a decade ago, allowed for large-scale protein profiling studies that have been applied in the identification of distinctive molecular cell signatures. Proteomics provides a powerful approach for identifying and studying these multiple molecular markers in a vast array of biological systems, whether focusing on basic biological research, diagnosis, therapeutics, or systems biology. This is a continuously expanding field that relies on the combination of different methodologies and current advances, both technological and analytical, which have led to an explosion of protein signatures and biomarker candidates. But how are these biological markers obtained? And, most importantly, what can we learn from them? Herein, we briefly overview the currently available approaches for obtaining relevant information at the proteome level, while noting the current and future roles of both traditional and modern proteomics. Moreover, we provide some considerations on how the development of powerful and robust bioinformatics tools will greatly benefit high-throughput proteomics. Such strategies are of the utmost importance in the rapidly emerging field of immunoproteomics, which may play a key role in the identification of antigens with diagnostic and/or therapeutic potential and in the development of new vaccines. Finally, we consider the present limitations in the discovery of new signatures and biomarkers and speculate on how such hurdles may be overcome, while also offering a prospect for the next few years in what could be one of the most significant strategies in translational medicine research. Springer-Verlag Berlin Heidelberg, 2015 Proteome signatures nationallicence Biomarkers nationallicence Proteomics nationallicence Immunoproteomics nationallicence da Costa João Mass Spectrometry Centre, QOPNA, Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Aveiro, Portugal aut Carvalhais Virginia Mass Spectrometry Centre, QOPNA, Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Aveiro, Portugal aut Ferreira Rita Mass Spectrometry Centre, QOPNA, Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Aveiro, Portugal aut Amado Francisco Mass Spectrometry Centre, QOPNA, Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Aveiro, Portugal aut Vilanova Manuel IBMC - Instituto de Biologia Molecular e Celular, Rua do Campo Alegre 83, Porto, Portugal aut Cerca Nuno CEB - Centre of Biological Engineering, LIBRO - Laboratory of Research in Biofilms Rosário Oliveira, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal aut Vitorino Rui Mass Spectrometry Centre, QOPNA, Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Aveiro, Portugal aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/18(2015-09-01), 7417-7431 0175-7598 99:18<7417 2015 99 253 https://doi.org/10.1007/s00253-015-6795-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6795-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- da Costa João Mass Spectrometry Centre, QOPNA, Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Aveiro, Portugal aut NATIONALLICENCE 700 1- Carvalhais Virginia Mass Spectrometry Centre, QOPNA, Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Aveiro, Portugal aut NATIONALLICENCE 700 1- Ferreira Rita Mass Spectrometry Centre, QOPNA, Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Aveiro, Portugal aut NATIONALLICENCE 700 1- Amado Francisco Mass Spectrometry Centre, QOPNA, Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Aveiro, Portugal aut NATIONALLICENCE 700 1- Vilanova Manuel IBMC - Instituto de Biologia Molecular e Celular, Rua do Campo Alegre 83, Porto, Portugal aut NATIONALLICENCE 700 1- Cerca Nuno CEB - Centre of Biological Engineering, LIBRO - Laboratory of Research in Biofilms Rosário Oliveira, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal aut NATIONALLICENCE 700 1- Vitorino Rui Mass Spectrometry Centre, QOPNA, Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Aveiro, Portugal aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/18(2015-09-01), 7417-7431 0175-7598 99:18<7417 2015 99 253