caa a22 4500 605501491 CHVBK 20210128100601.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00253-015-6627-9 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6627-9 Community shifts in a well-operating agricultural biogas plant: how process variations are handled by the microbiome [Elektronische Daten] [Susanne Theuerl, Fabian Kohrs, Dirk Benndorf, Irena Maus, Daniel Wibberg, Andreas Schlüter, Robert Kausmann, Monika Heiermann, Erdmann Rapp, Udo Reichl, Alfred Pühler, Michael Klocke] This study provides a comprehensive, long-term microbiological study of a continuously operated, mesophilic, agricultural biogas plant fed with whole-crop silages of maize and rye, cattle manure and cattle slurry. The microbial community structure was accessed by high-throughput 16S rRNA gene amplicon sequencing. For the characterisation of the microbial dynamics, the community profiling method terminal restriction fragment length polymorphism (TRFLP) in combination with a cloning-sequencing approach as well as a LC-MS/MS approach for protein identification were applied. Our results revealed that the anaerobic digestion is a highly sensitive process: small variations in the process performance induce fluctuations in the microbial community composition and activity. In this context, it could be proven that certain microbial species were better adapted to changing process condition such as temperature (interspecies competition) and that there is a physiological compensation between different microorganisms so that the reactor efficiency was not adversely affected despite of structural and functional changes within the microbial community. Springer-Verlag Berlin Heidelberg, 2015 Full-scale (agricultural) biogas plant nationallicence Microbial community nationallicence 16S rRNA gene amplicon sequencing nationallicence TRFLP nationallicence Metaproteomic analysis nationallicence Ecosystem fluctuations and stability nationallicence Theuerl Susanne Department Bioengineering, Leibniz-Institute for Agricultural Engineering Potsdam-Bornim e.V. (ATB), Max-Eyth-Allee 100, 14469, Potsdam, Germany aut Kohrs Fabian Bioprocess Engineering, Otto von Guericke University Magdeburg, Universitätsplatz 2, 39106, Magdeburg, Germany aut Benndorf Dirk Bioprocess Engineering, Otto von Guericke University Magdeburg, Universitätsplatz 2, 39106, Magdeburg, Germany aut Maus Irena Institute for Genome Research and System Biology, Center for Biotechnology (CeBiTec), Universitätsstraße 27, 33615, Bielefeld, Germany aut Wibberg Daniel Institute for Genome Research and System Biology, Center for Biotechnology (CeBiTec), Universitätsstraße 27, 33615, Bielefeld, Germany aut Schlüter Andreas Institute for Genome Research and System Biology, Center for Biotechnology (CeBiTec), Universitätsstraße 27, 33615, Bielefeld, Germany aut Kausmann Robert Department Technology Assessment and Substance Cycles, Leibniz-Institute for Agricultural Engineering Potsdam-Bornim e.V., Max-Eyth-Allee 100, 14469, Potsdam, Germany aut Heiermann Monika Department Technology Assessment and Substance Cycles, Leibniz-Institute for Agricultural Engineering Potsdam-Bornim e.V., Max-Eyth-Allee 100, 14469, Potsdam, Germany aut Rapp Erdmann Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstraße 1, 39106, Magdeburg, Germany aut Reichl Udo Bioprocess Engineering, Otto von Guericke University Magdeburg, Universitätsplatz 2, 39106, Magdeburg, Germany aut Pühler Alfred Institute for Genome Research and System Biology, Center for Biotechnology (CeBiTec), Universitätsstraße 27, 33615, Bielefeld, Germany aut Klocke Michael Department Bioengineering, Leibniz-Institute for Agricultural Engineering Potsdam-Bornim e.V. (ATB), Max-Eyth-Allee 100, 14469, Potsdam, Germany aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/18(2015-09-01), 7791-7803 0175-7598 99:18<7791 2015 99 253 https://doi.org/10.1007/s00253-015-6627-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6627-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Theuerl Susanne Department Bioengineering, Leibniz-Institute for Agricultural Engineering Potsdam-Bornim e.V. (ATB), Max-Eyth-Allee 100, 14469, Potsdam, Germany aut NATIONALLICENCE 700 1- Kohrs Fabian Bioprocess Engineering, Otto von Guericke University Magdeburg, Universitätsplatz 2, 39106, Magdeburg, Germany aut NATIONALLICENCE 700 1- Benndorf Dirk Bioprocess Engineering, Otto von Guericke University Magdeburg, Universitätsplatz 2, 39106, Magdeburg, Germany aut NATIONALLICENCE 700 1- Maus Irena Institute for Genome Research and System Biology, Center for Biotechnology (CeBiTec), Universitätsstraße 27, 33615, Bielefeld, Germany aut NATIONALLICENCE 700 1- Wibberg Daniel Institute for Genome Research and System Biology, Center for Biotechnology (CeBiTec), Universitätsstraße 27, 33615, Bielefeld, Germany aut NATIONALLICENCE 700 1- Schlüter Andreas Institute for Genome Research and System Biology, Center for Biotechnology (CeBiTec), Universitätsstraße 27, 33615, Bielefeld, Germany aut NATIONALLICENCE 700 1- Kausmann Robert Department Technology Assessment and Substance Cycles, Leibniz-Institute for Agricultural Engineering Potsdam-Bornim e.V., Max-Eyth-Allee 100, 14469, Potsdam, Germany aut NATIONALLICENCE 700 1- Heiermann Monika Department Technology Assessment and Substance Cycles, Leibniz-Institute for Agricultural Engineering Potsdam-Bornim e.V., Max-Eyth-Allee 100, 14469, Potsdam, Germany aut NATIONALLICENCE 700 1- Rapp Erdmann Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstraße 1, 39106, Magdeburg, Germany aut NATIONALLICENCE 700 1- Reichl Udo Bioprocess Engineering, Otto von Guericke University Magdeburg, Universitätsplatz 2, 39106, Magdeburg, Germany aut NATIONALLICENCE 700 1- Pühler Alfred Institute for Genome Research and System Biology, Center for Biotechnology (CeBiTec), Universitätsstraße 27, 33615, Bielefeld, Germany aut NATIONALLICENCE 700 1- Klocke Michael Department Bioengineering, Leibniz-Institute for Agricultural Engineering Potsdam-Bornim e.V. (ATB), Max-Eyth-Allee 100, 14469, Potsdam, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/18(2015-09-01), 7791-7803 0175-7598 99:18<7791 2015 99 253