Metabolic engineering for improved production of ethanol by Corynebacterium glutamicum
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6223-4 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6223-4 | ||
| 245 | 0 | 0 | |a Metabolic engineering for improved production of ethanol by Corynebacterium glutamicum |h [Elektronische Daten] |c [Toru Jojima, Ryoji Noburyu, Miho Sasaki, Takahisa Tajima, Masako Suda, Hideaki Yukawa, Masayuki Inui] |
| 520 | 3 | |a Recombinant Corynebacterium glutamicum harboring genes for pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adhB) can produce ethanol under oxygen deprivation. We investigated the effects of elevating the expression levels of glycolytic genes, as well as pdc and adhB, on ethanol production. Overexpression of four glycolytic genes (pgi, pfkA, gapA, and pyk) in C. glutamicum significantly increased the rate of ethanol production. Overexpression of tpi, encoding triosephosphate isomerase, further enhanced productivity. Elevated expression of pdc and adhB increased ethanol yield, but not the rate of production. Fed-batch fermentation using an optimized strain resulted in ethanol production of 119g/L from 245g/L glucose with a yield of 95% of the theoretical maximum. Further metabolic engineering, including integration of the genes for xylose and arabinose metabolism, enabled consumption of glucose, xylose, and arabinose, and ethanol production (83g/L) at a yield of 90%. This study demonstrated that C. glutamicum has significant potential for the production of cellulosic ethanol. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Corynebacterium glutamicum |2 nationallicence | |
| 690 | 7 | |a Oxygen deprivation |2 nationallicence | |
| 690 | 7 | |a Ethanol |2 nationallicence | |
| 690 | 7 | |a Glycolytic enzymes |2 nationallicence | |
| 690 | 7 | |a Mixed sugars |2 nationallicence | |
| 700 | 1 | |a Jojima |D Toru |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | |
| 700 | 1 | |a Noburyu |D Ryoji |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | |
| 700 | 1 | |a Sasaki |D Miho |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | |
| 700 | 1 | |a Tajima |D Takahisa |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | |
| 700 | 1 | |a Suda |D Masako |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | |
| 700 | 1 | |a Yukawa |D Hideaki |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | |
| 700 | 1 | |a Inui |D Masayuki |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/3(2015-02-01), 1165-1172 |x 0175-7598 |q 99:3<1165 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6223-4 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6223-4 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Jojima |D Toru |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Noburyu |D Ryoji |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sasaki |D Miho |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tajima |D Takahisa |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Suda |D Masako |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yukawa |D Hideaki |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Inui |D Masayuki |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/3(2015-02-01), 1165-1172 |x 0175-7598 |q 99:3<1165 |1 2015 |2 99 |o 253 | ||