caa a22 4500 605501602 CHVBK 20210128100601.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-6223-4 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6223-4 Metabolic engineering for improved production of ethanol by Corynebacterium glutamicum [Elektronische Daten] [Toru Jojima, Ryoji Noburyu, Miho Sasaki, Takahisa Tajima, Masako Suda, Hideaki Yukawa, Masayuki Inui] Recombinant Corynebacterium glutamicum harboring genes for pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adhB) can produce ethanol under oxygen deprivation. We investigated the effects of elevating the expression levels of glycolytic genes, as well as pdc and adhB, on ethanol production. Overexpression of four glycolytic genes (pgi, pfkA, gapA, and pyk) in C. glutamicum significantly increased the rate of ethanol production. Overexpression of tpi, encoding triosephosphate isomerase, further enhanced productivity. Elevated expression of pdc and adhB increased ethanol yield, but not the rate of production. Fed-batch fermentation using an optimized strain resulted in ethanol production of 119g/L from 245g/L glucose with a yield of 95% of the theoretical maximum. Further metabolic engineering, including integration of the genes for xylose and arabinose metabolism, enabled consumption of glucose, xylose, and arabinose, and ethanol production (83g/L) at a yield of 90%. This study demonstrated that C. glutamicum has significant potential for the production of cellulosic ethanol. Springer-Verlag Berlin Heidelberg, 2014 Corynebacterium glutamicum nationallicence Oxygen deprivation nationallicence Ethanol nationallicence Glycolytic enzymes nationallicence Mixed sugars nationallicence Jojima Toru Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut Noburyu Ryoji Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut Sasaki Miho Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut Tajima Takahisa Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut Suda Masako Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut Yukawa Hideaki Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut Inui Masayuki Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1165-1172 0175-7598 99:3<1165 2015 99 253 https://doi.org/10.1007/s00253-014-6223-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6223-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jojima Toru Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut NATIONALLICENCE 700 1- Noburyu Ryoji Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut NATIONALLICENCE 700 1- Sasaki Miho Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut NATIONALLICENCE 700 1- Tajima Takahisa Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut NATIONALLICENCE 700 1- Suda Masako Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut NATIONALLICENCE 700 1- Yukawa Hideaki Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut NATIONALLICENCE 700 1- Inui Masayuki Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1165-1172 0175-7598 99:3<1165 2015 99 253