caa a22 4500 605501645 CHVBK 20210128100601.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-6227-0 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6227-0 Accumulation of yellow Monascus pigments by extractive fermentation in nonionic surfactant micelle aqueous solution [Elektronische Daten] [Xu Xiong, Xuehong Zhang, Zhenqiang Wu, Zhilong Wang] Monascus species can produce various secondary metabolites of polyketide structure. In the current study, it is found that an interesting phenomenon, i.e., submerged culture of Monascus species in an aqueous solution majorly accumulated intracellular orange Monascus pigments exhibiting one peak at 470nm with absorbance of 32 OD while extractive fermentation in a nonionic surfactant micelle aqueous solution produced extracellular and intracellular yellow Monascus pigments exhibiting one peak at 410nm with absorbance 30 OD and 12 OD, respectively. The spectrum profiles of both intracellular and extracellular Monascus pigments were affected by surfactant loading, extractive fermentation time, and surfactant adding time. Meanwhile, the instability of orange Monascus pigments in the extracellular nonionic surfactant micelle aqueous solution was also confirmed experimentally. The mechanism behind this phenomenon is attributed to the export of intracellular yellow Monascus pigments into its broth by extractive fermentation. The transferring of intracellular yellow Monascus pigments into its broth blocks yellow Monascus pigments from further enzymatic conversion or eliminates the feedback inhibition of yellow Monascus pigments based on the biosynthetic pathway of Monascus pigments. Springer-Verlag Berlin Heidelberg, 2014 Nonionic surfactant nationallicence Extractive fermentation nationallicence Monascus pigments nationallicence Yellow colorant nationallicence Xiong Xu School of Pharmacy, and State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut Zhang Xuehong School of Life Science and Biotechnology, and State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut Wu Zhenqiang School of Biological Science and Engineering, South China University of Technology, 510006, Guangzhou, People's Republic of China aut Wang Zhilong School of Pharmacy, and State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1173-1180 0175-7598 99:3<1173 2015 99 253 https://doi.org/10.1007/s00253-014-6227-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6227-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xiong Xu School of Pharmacy, and State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Xuehong School of Life Science and Biotechnology, and State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Wu Zhenqiang School of Biological Science and Engineering, South China University of Technology, 510006, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Wang Zhilong School of Pharmacy, and State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1173-1180 0175-7598 99:3<1173 2015 99 253