caa a22 4500 60550167X CHVBK 20210128100602.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-6133-5 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6133-5 A yeast pheromone-based inter-species communication system [Elektronische Daten] [Stefan Hennig, André Clemens, Gerhard Rödel, Kai Ostermann] We report on a pheromone-based inter-species communication system, allowing for a controlled cell-cell communication between the two species Saccharomyces cerevisiae and Schizosaccharomyces pombe as a proof of principle. It exploits the mating response pathways of the two yeast species employing the pheromones, α- or P-factor, as signaling molecules. The authentic and chimeric pheromone-encoding genes were engineered to code for the P-factor in S. cerevisiae and the α-factor in S. pombe. Upon transformation of the respective constructs, cells were enabled to express the mating pheromone of the opposite species. The supernatant of cultures of S. pombe cells expressing α-factor were able to induce a G1 arrest in the cell cycle, a change in morphology to the typical shmoo effect and expression driven by the pheromone-responsive FIG1 promoter in S. cerevisiae. The supernatant of cultures of S. cerevisiae cells expressing P-factor similarly induced cell cycle arrest in G1, an alteration in morphology typical for mating as well as the activation of the pheromone-responsive promoters of the rep1 and sxa2 genes in a pheromone-hypersensitive reporter strain of S. pombe. Apparently, both heterologous pheromones were correctly processed and secreted in an active form by the cells of the other species. Our data clearly show that the species-specific pheromone systems of yeast species can be exploited for a controlled inter-species communication. Springer-Verlag Berlin Heidelberg, 2014 Pheromone nationallicence Yeast nationallicence Inter-species communication nationallicence S. cerevisiae nationallicence S. pombe nationallicence Synthetic biology nationallicence Hennig Stefan Institut für Genetik, Technische Universität Dresden, 01062, Dresden, Germany aut Clemens André Institut für Genetik, Technische Universität Dresden, 01062, Dresden, Germany aut Rödel Gerhard Institut für Genetik, Technische Universität Dresden, 01062, Dresden, Germany aut Ostermann Kai Institut für Genetik, Technische Universität Dresden, 01062, Dresden, Germany aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1299-1308 0175-7598 99:3<1299 2015 99 253 https://doi.org/10.1007/s00253-014-6133-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6133-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hennig Stefan Institut für Genetik, Technische Universität Dresden, 01062, Dresden, Germany aut NATIONALLICENCE 700 1- Clemens André Institut für Genetik, Technische Universität Dresden, 01062, Dresden, Germany aut NATIONALLICENCE 700 1- Rödel Gerhard Institut für Genetik, Technische Universität Dresden, 01062, Dresden, Germany aut NATIONALLICENCE 700 1- Ostermann Kai Institut für Genetik, Technische Universität Dresden, 01062, Dresden, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1299-1308 0175-7598 99:3<1299 2015 99 253