caa a22 4500 605501696 CHVBK 20210128100602.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-6058-z doi (NATIONALLICENCE)springer-10.1007/s00253-014-6058-z Molecular analysis of methanogens involved in methanogenic degradation of tetramethylammonium hydroxide in full-scale bioreactors [Elektronische Daten] [Liang-Ming Whang, Tai-Ho Hu, Pao-Wen Liu, Yu-Ching Hung, Toshikazu Fukushima, Yi-Ju Wu, Shao-Hsiung Chang] This study investigated methanogenic communities involved in degradation of tetramethylammonium hydroxide (TMAH) in three full-scale bioreactors treating TMAH-containing wastewater. Based on the results of terminal-restriction fragment-length polymorphism (T-RFLP) and quantitative PCR analyses targeting the methyl-coenzyme M reductase alpha subunit (mcrA) genes retrieved from three bioreactors, Methanomethylovorans and Methanosarcina were the dominant methanogens involved in the methanogenic degradation of TMAH in the bioreactors. Furthermore, batch experiments were conducted to evaluate mcrA messenger RNA (mRNA) expression during methanogenic TMAH degradation, and the results indicated that a higher level of TMAH favored mcrA mRNA expression by Methansarcina, while Methanomethylovorans could only express considerable amount of mcrA mRNA at a lower level of TMAH. These results suggest that Methansarcina is responsible for methanogenic TMAH degradation at higher TMAH concentrations, while Methanomethylovorans may be important at a lower TMAH condition. Springer-Verlag Berlin Heidelberg, 2014 Anaerobic wastewater treatment nationallicence mcrA nationallicence mRNA expression nationallicence Methanomethylovorans nationallicence Methanosarcina nationallicence Whang Liang-Ming Department of Environmental Engineering, National Cheng Kung University, No. 1, University Road, 701, Tainan, Taiwan, Republic of China aut Hu Tai-Ho Department of Environmental Engineering, National Cheng Kung University, No. 1, University Road, 701, Tainan, Taiwan, Republic of China aut Liu Pao-Wen Department of Safety Health and Environmental Engineering, Chung Hwa University of Medical Technology, Tainan, Taiwan, Republic of China aut Hung Yu-Ching Department of Environmental Engineering, National Cheng Kung University, No. 1, University Road, 701, Tainan, Taiwan, Republic of China aut Fukushima Toshikazu Department of Environmental Engineering, National Cheng Kung University, No. 1, University Road, 701, Tainan, Taiwan, Republic of China aut Wu Yi-Ju Department of Environmental Engineering, National Cheng Kung University, No. 1, University Road, 701, Tainan, Taiwan, Republic of China aut Chang Shao-Hsiung Department of Environmental Engineering, National Cheng Kung University, No. 1, University Road, 701, Tainan, Taiwan, Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1485-1497 0175-7598 99:3<1485 2015 99 253 https://doi.org/10.1007/s00253-014-6058-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6058-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Whang Liang-Ming Department of Environmental Engineering, National Cheng Kung University, No. 1, University Road, 701, Tainan, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Hu Tai-Ho Department of Environmental Engineering, National Cheng Kung University, No. 1, University Road, 701, Tainan, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Liu Pao-Wen Department of Safety Health and Environmental Engineering, Chung Hwa University of Medical Technology, Tainan, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Hung Yu-Ching Department of Environmental Engineering, National Cheng Kung University, No. 1, University Road, 701, Tainan, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Fukushima Toshikazu Department of Environmental Engineering, National Cheng Kung University, No. 1, University Road, 701, Tainan, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Wu Yi-Ju Department of Environmental Engineering, National Cheng Kung University, No. 1, University Road, 701, Tainan, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Chang Shao-Hsiung Department of Environmental Engineering, National Cheng Kung University, No. 1, University Road, 701, Tainan, Taiwan, Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1485-1497 0175-7598 99:3<1485 2015 99 253