caa a22 4500 605501718 CHVBK 20210128100602.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-6180-y doi (NATIONALLICENCE)springer-10.1007/s00253-014-6180-y Manipulation of pyrite colonization and leaching by iron-oxidizing Acidithiobacillus species [Elektronische Daten] [Sören Bellenberg, Robert Barthen, Mariia Boretska, Ruiyong Zhang, Wolfgang Sand, Mario Vera] In this study, the process of pyrite colonization and leaching by three iron-oxidizing Acidithiobacillus species was investigated by fluorescence microscopy, bacterial attachment, and leaching assays. Within the first 4-5days, only the biofilm subpopulation was responsible for pyrite dissolution. Pyrite-grown cells, in contrast to iron-grown cells, were able to oxidize iron(II) ions or pyrite after 24 h iron starvation and incubation with 1mM H2O2, indicating that these cells were adapted to the presence of enhanced levels of reactive oxygen species (ROS), which are generated on metal sulfide surfaces. Acidithiobacillus ferrivorans SS3 and Acidithiobacillus ferrooxidans R1 showed enhanced pyrite colonization and biofilm formation compared to A. ferrooxidans T. A broad range of factors influencing the biofilm formation on pyrite were also identified, some of them were strain-specific. Cultivation at non-optimum growth temperatures or increased ionic strength led to a decreased colonization of pyrite. The presence of iron(III) ions increased pyrite colonization, especially when pyrite-grown cells were used, while the addition of 20mM copper(II) ions resulted in reduced biofilm formation on pyrite. This observation correlated with a different extracellular polymeric substance (EPS) composition of copper-exposed cells. Interestingly, the addition of 1mM sodium glucuronate in combination with iron(III) ions led to a 5-fold and 7-fold increased cell attachment after 1 and 8days of incubation, respectively, in A. ferrooxidans T. In addition, sodium glucuronate addition enhanced pyrite dissolution by 25%. Springer-Verlag Berlin Heidelberg, 2014 Attachment nationallicence Biofilms nationallicence Pyrite dissolution nationallicence EPS nationallicence Fluorescence microscopy nationallicence Bellenberg Sören Fakultät für Chemie, Biofilm Centre, Aquatische Biotechnologie, Universität Duisburg-Essen Universitätsstr 5, 45141, Essen, Germany aut Barthen Robert Fakultät für Chemie, Biofilm Centre, Aquatische Biotechnologie, Universität Duisburg-Essen Universitätsstr 5, 45141, Essen, Germany aut Boretska Mariia National Academy of Sciences of Ukraine, D.K. Zabolotny Institute of Microbiology and Virology, Zabolotnogo Str. 154, DO3680 MSP, Kiev, Ukraine aut Zhang Ruiyong Fakultät für Chemie, Biofilm Centre, Aquatische Biotechnologie, Universität Duisburg-Essen Universitätsstr 5, 45141, Essen, Germany aut Sand Wolfgang Fakultät für Chemie, Biofilm Centre, Aquatische Biotechnologie, Universität Duisburg-Essen Universitätsstr 5, 45141, Essen, Germany aut Vera Mario Fakultät für Chemie, Biofilm Centre, Aquatische Biotechnologie, Universität Duisburg-Essen Universitätsstr 5, 45141, Essen, Germany aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1435-1449 0175-7598 99:3<1435 2015 99 253 https://doi.org/10.1007/s00253-014-6180-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6180-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bellenberg Sören Fakultät für Chemie, Biofilm Centre, Aquatische Biotechnologie, Universität Duisburg-Essen Universitätsstr 5, 45141, Essen, Germany aut NATIONALLICENCE 700 1- Barthen Robert Fakultät für Chemie, Biofilm Centre, Aquatische Biotechnologie, Universität Duisburg-Essen Universitätsstr 5, 45141, Essen, Germany aut NATIONALLICENCE 700 1- Boretska Mariia National Academy of Sciences of Ukraine, D.K. Zabolotny Institute of Microbiology and Virology, Zabolotnogo Str. 154, DO3680 MSP, Kiev, Ukraine aut NATIONALLICENCE 700 1- Zhang Ruiyong Fakultät für Chemie, Biofilm Centre, Aquatische Biotechnologie, Universität Duisburg-Essen Universitätsstr 5, 45141, Essen, Germany aut NATIONALLICENCE 700 1- Sand Wolfgang Fakultät für Chemie, Biofilm Centre, Aquatische Biotechnologie, Universität Duisburg-Essen Universitätsstr 5, 45141, Essen, Germany aut NATIONALLICENCE 700 1- Vera Mario Fakultät für Chemie, Biofilm Centre, Aquatische Biotechnologie, Universität Duisburg-Essen Universitätsstr 5, 45141, Essen, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1435-1449 0175-7598 99:3<1435 2015 99 253