caa a22 4500 605501726 CHVBK 20210128100602.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-6198-1 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6198-1 Cellulose production and cellulose synthase gene detection in acetic acid bacteria [Elektronische Daten] [Maria Valera, Maria Torija, Albert Mas, Estibaliz Mateo] The ability of acetic acid bacteria (AAB) to produce cellulose has gained much industrial interest due to the physical and chemical characteristics of bacterial cellulose. The production of cellulose occurs in the presence of oxygen and in a glucose-containing medium, but it can also occur during vinegar elaboration by the traditional method. The vinegar biofilm produced by AAB on the air-liquid interface is primarily composed of cellulose and maintains the cells in close contact with oxygen. In this study, we screened for the ability of AAB to produce cellulose using different carbon sources in the presence or absence of ethanol. The presence of cellulose in biofilms was confirmed using the fluorochrome Calcofluor by microscopy. Moreover, the process of biofilm formation was monitored under epifluorescence microscopy using the Live/Dead BacLight Kit. A total of 77 AAB strains belonging to 35 species of Acetobacter, Komagataeibacter, Gluconacetobacter, and Gluconobacter were analysed, and 30 strains were able to produce a cellulose biofilm in at least one condition. This cellulose production was correlated with the PCR amplification of the bcsA gene that encodes cellulose synthase. A total of eight degenerated primers were designed, resulting in one primer pair that was able to detect the presence of this gene in 27 AAB strains, 26 of which formed cellulose. Springer-Verlag Berlin Heidelberg, 2014 Vinegar nationallicence Biofilm nationallicence Epifluorescence microscopy nationallicence bcsA gene nationallicence Valera Maria Biotecnologia Enológica. Dept. Bioquímica i Biotecnologia, Facultat d‘Enologia, Universitat Rovira i Virgili, C/ Marcel.lí Domingo s/n., 43007, Tarragona, Spain aut Torija Maria Biotecnologia Enológica. Dept. Bioquímica i Biotecnologia, Facultat d‘Enologia, Universitat Rovira i Virgili, C/ Marcel.lí Domingo s/n., 43007, Tarragona, Spain aut Mas Albert Biotecnologia Enológica. Dept. Bioquímica i Biotecnologia, Facultat d‘Enologia, Universitat Rovira i Virgili, C/ Marcel.lí Domingo s/n., 43007, Tarragona, Spain aut Mateo Estibaliz Departamento de Inmunología, Microbiología y Parasitología, Facultad de Medicina y Odontología, Universidad del País Vasco/Euskal Herriko Unibertsitatea, UPV/EHU, Barrio Sarriena s/n., 48940, Leioa, Spain aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1349-1361 0175-7598 99:3<1349 2015 99 253 https://doi.org/10.1007/s00253-014-6198-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6198-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Valera Maria Biotecnologia Enológica. Dept. Bioquímica i Biotecnologia, Facultat d‘Enologia, Universitat Rovira i Virgili, C/ Marcel.lí Domingo s/n., 43007, Tarragona, Spain aut NATIONALLICENCE 700 1- Torija Maria Biotecnologia Enológica. Dept. Bioquímica i Biotecnologia, Facultat d‘Enologia, Universitat Rovira i Virgili, C/ Marcel.lí Domingo s/n., 43007, Tarragona, Spain aut NATIONALLICENCE 700 1- Mas Albert Biotecnologia Enológica. Dept. Bioquímica i Biotecnologia, Facultat d‘Enologia, Universitat Rovira i Virgili, C/ Marcel.lí Domingo s/n., 43007, Tarragona, Spain aut NATIONALLICENCE 700 1- Mateo Estibaliz Departamento de Inmunología, Microbiología y Parasitología, Facultad de Medicina y Odontología, Universidad del País Vasco/Euskal Herriko Unibertsitatea, UPV/EHU, Barrio Sarriena s/n., 48940, Leioa, Spain aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1349-1361 0175-7598 99:3<1349 2015 99 253