caa a22 4500 605501777 CHVBK 20210128100602.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-6162-0 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6162-0 A novel hybrid baculovirus-adeno-associated viral vector-mediated radionuclide reporter gene imaging system for stem cells transplantation monitoring [Elektronische Daten] [Yu Pan, Hongyan Yin, Jing Lv, Huijun Ju, Xiang Zhou, Yifan Zhang] Hybrid baculovirus-adeno-associated virus (BV-AAV) containing enhanced green fluorescent protein (eGFP) reporter gene or human sodium-iodide symporter (hNIS) reporter gene flanked by inverted terminal repeats (ITRs) derived from AAV (BV-CMV-eGFP-ITR and BV-CMV-hNIS-ITR) were constructed and used to investigate the feasibility of using hybrid BV-AAV transgenic vector to mediate hNIS reporter gene imaging for monitoring bone marrow-derived mesenchymal stem cells (BM-MSCs) transplantation therapy as a novel biotechnological platform in radionuclide reporter geneimaging. The results showed that the infection efficiency of BV-CMV-eGFP-ITR in BM-MSCs reached 84.25 ± 1.38%, and there were no obvious adverse effects on BM-MSCs. The 125I− and 99mTcO4 − uptake assays showed that the radionuclide accumulation induced by BA-AAV-mediated hNIS was highly efficient in infected BM-MSCs. Furthermore, there was a robust correlation between the infected BM-MSCs cell number and the 125I− accumulation amount (R 2 = 0.9026). The micro-SPECT/CT imaging showed that BV-CMV-hNIS-ITR-infected BM-MSCs accumulated radioiodine efficiently in vivo, exhibiting obvious radiotracer accumulation in transplantation sites. Further quantitative analysis revealed that 30min might be the optimal imaging time point. Moreover, the revealed high target/individual organ background ratios also supported the feasibility of BV-AAV-mediated hNIS reporter gene imaging for monitoring BM-MSCs transplantation in most of commonly used transplantation sites, thus highlighting this promise biotechnological platform in radionuclide reporter gene imaging for stem cell transplantation therapy. Springer-Verlag Berlin Heidelberg, 2014 Baculovirus nationallicence Hybrid virus nationallicence Inverted terminal repeat nationallicence Mesenchymal stem cells nationallicence Radionuclide reporter gene nationallicence Sodium-iodine symporter nationallicence Pan Yu Department of Nuclear Medicine, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197, Ruijin 2nd Road, 200025, Shanghai, China aut Yin Hongyan Department of Nuclear Medicine, Zhong Shan Hospital, Fudan University, No. 180, Fenglin Road, 200032, Shanghai, China aut Lv Jing Department of Nuclear Medicine, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197, Ruijin 2nd Road, 200025, Shanghai, China aut Ju Huijun Department of Nuclear Medicine, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197, Ruijin 2nd Road, 200025, Shanghai, China aut Zhou Xiang Department of Nuclear Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, No. 1630, Dongfang Road, 200127, Shanghai, China aut Zhang Yifan Department of Nuclear Medicine, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197, Ruijin 2nd Road, 200025, Shanghai, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1415-1426 0175-7598 99:3<1415 2015 99 253 https://doi.org/10.1007/s00253-014-6162-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6162-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pan Yu Department of Nuclear Medicine, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197, Ruijin 2nd Road, 200025, Shanghai, China aut NATIONALLICENCE 700 1- Yin Hongyan Department of Nuclear Medicine, Zhong Shan Hospital, Fudan University, No. 180, Fenglin Road, 200032, Shanghai, China aut NATIONALLICENCE 700 1- Lv Jing Department of Nuclear Medicine, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197, Ruijin 2nd Road, 200025, Shanghai, China aut NATIONALLICENCE 700 1- Ju Huijun Department of Nuclear Medicine, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197, Ruijin 2nd Road, 200025, Shanghai, China aut NATIONALLICENCE 700 1- Zhou Xiang Department of Nuclear Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, No. 1630, Dongfang Road, 200127, Shanghai, China aut NATIONALLICENCE 700 1- Zhang Yifan Department of Nuclear Medicine, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197, Ruijin 2nd Road, 200025, Shanghai, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1415-1426 0175-7598 99:3<1415 2015 99 253