caa a22 4500 605501807 CHVBK 20210128100602.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-6270-x doi (NATIONALLICENCE)springer-10.1007/s00253-014-6270-x Improving mycoinsecticides for insect biological control [Elektronische Daten] [Almudena Ortiz-Urquiza, Zhibing Luo, Nemat Keyhani] The desire for decreased reliance on chemical pesticides continues to fuel interest in alternative means for pest control including the use of naturally occurring microbial insect pathogens. Insects, as vectors of disease causing agents or as agricultural pests, are responsible for millions of deaths and significant economic losses worldwide, placing stresses on productivity (GDP) and human health and welfare. In addition, alterations in climate change are likely to affect insect ranges, expanding their access to previously constrained geographic areas, a potentially worrisome outcome. Metarhizium anisopliae and Beauveria bassiana, two cosmopolitan fungal pathogens of insects found in almost all ecosystems, are the most commonly applied mycoinsecticides for a variety of insect control purposes. The availability of the complete genomes for both organisms coupled to robust technologies for their transformation has led to several advances in engineering these fungi for greater efficacy and/or utility in pest control applications. Here, we will provide an overview of the fungal-insect and fungal-plant interactions that occur and highlight recent advances in the genetic engineering of these fungi. The latter work has resulted in the development of strains displaying (1) increased resistance to abiotic stress, (2) increased cuticular targeting and degradation, (3) increased virulence via expression of insecticidal protein/peptide toxins, (4) the ability to block transmission of disease causing agents, and (5) the ability to target specific insect hosts, decrease host fecundity, and/or alter insect behaviors. Springer-Verlag Berlin Heidelberg, 2014 Pest control nationallicence Entomopathogenic fungi nationallicence Genetic engineering nationallicence Fungal development nationallicence Virulence nationallicence Stress response nationallicence Ortiz-Urquiza Almudena Department of Microbiology and Cell Science, University of Florida, 32611, Gainesville, FL, USA aut Luo Zhibing Department of Microbiology and Cell Science, University of Florida, 32611, Gainesville, FL, USA aut Keyhani Nemat Department of Microbiology and Cell Science, University of Florida, 32611, Gainesville, FL, USA aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1057-1068 0175-7598 99:3<1057 2015 99 253 https://doi.org/10.1007/s00253-014-6270-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6270-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ortiz-Urquiza Almudena Department of Microbiology and Cell Science, University of Florida, 32611, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Luo Zhibing Department of Microbiology and Cell Science, University of Florida, 32611, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Keyhani Nemat Department of Microbiology and Cell Science, University of Florida, 32611, Gainesville, FL, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1057-1068 0175-7598 99:3<1057 2015 99 253