Linear B-cell epitope mapping of MAPK3 and MAPK4 from Leishmania braziliensis
implications for the serodiagnosis of human and canine leishmaniasis
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6168-7 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6168-7 | ||
| 245 | 0 | 0 | |a Linear B-cell epitope mapping of MAPK3 and MAPK4 from Leishmania braziliensis |h [Elektronische Daten] |b implications for the serodiagnosis of human and canine leishmaniasis |c [Daniel Menezes-Souza, Tiago de Oliveira Mendes, Ana de Araújo Leão, Matheus de Souza Gomes, Ricardo Fujiwara, Daniella Bartholomeu] |
| 520 | 3 | |a The correct and early identification of humans and dogs infected with Leishmania are key steps in the control of leishmaniasis. Additionally, a method with high sensitivity and specificity at low cost that allows the screening of a large number of samples would be extremely valuable. In this study, we analyzed the potential of mitogen-activated protein kinase 3 (MAPK3) and mitogen-activated protein kinase 4 (MAPK4) proteins from Leishmania braziliensis to serve as antigen candidates for the serodiagnosis of human visceral and tegumentary leishmaniasis, as well as canine visceral disease. Moreover, we mapped linear B-cell epitopes in these proteins and selected those epitopes with sequences that were divergent in the corresponding orthologs in Homo sapiens, in Canis familiaris, and in Trypanosoma cruzi. We compared the performance of these peptides with the recombinant protein using ELISA. Both MAPK3 and MAPK4 recombinant proteins showed better specificity in the immunodiagnosis of human and canine leishmaniasis than soluble parasite antigens and the EIE-leishmaniose-visceral-canina-bio-manguinhos (EIE-LVC) kit. Furthermore, the performance of this serodiagnosis assay was improved using synthetic peptides corresponding to B-cell epitopes derived from both proteins. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Tegumentary and visceral human leishmaniasis |2 nationallicence | |
| 690 | 7 | |a Canine visceral leishmaniasis |2 nationallicence | |
| 690 | 7 | |a Immunodiagnosis |2 nationallicence | |
| 690 | 7 | |a MAPK3 |2 nationallicence | |
| 690 | 7 | |a MAPK4 |2 nationallicence | |
| 690 | 7 | |a B-cell epitope mapping |2 nationallicence | |
| 700 | 1 | |a Menezes-Souza |D Daniel |u Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil |4 aut | |
| 700 | 1 | |a de Oliveira Mendes |D Tiago |u Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil |4 aut | |
| 700 | 1 | |a de Araújo Leão |D Ana |u Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil |4 aut | |
| 700 | 1 | |a de Souza Gomes |D Matheus |u Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Patos de Minas, Minas Gerais, Brazil |4 aut | |
| 700 | 1 | |a Fujiwara |D Ricardo |u Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil |4 aut | |
| 700 | 1 | |a Bartholomeu |D Daniella |u Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/3(2015-02-01), 1323-1336 |x 0175-7598 |q 99:3<1323 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6168-7 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6168-7 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Menezes-Souza |D Daniel |u Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a de Oliveira Mendes |D Tiago |u Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a de Araújo Leão |D Ana |u Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a de Souza Gomes |D Matheus |u Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Patos de Minas, Minas Gerais, Brazil |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Fujiwara |D Ricardo |u Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bartholomeu |D Daniella |u Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/3(2015-02-01), 1323-1336 |x 0175-7598 |q 99:3<1323 |1 2015 |2 99 |o 253 | ||