caa a22 4500 605501823 CHVBK 20210128100602.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-6168-7 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6168-7 Linear B-cell epitope mapping of MAPK3 and MAPK4 from Leishmania braziliensis [Elektronische Daten] implications for the serodiagnosis of human and canine leishmaniasis [Daniel Menezes-Souza, Tiago de Oliveira Mendes, Ana de Araújo Leão, Matheus de Souza Gomes, Ricardo Fujiwara, Daniella Bartholomeu] The correct and early identification of humans and dogs infected with Leishmania are key steps in the control of leishmaniasis. Additionally, a method with high sensitivity and specificity at low cost that allows the screening of a large number of samples would be extremely valuable. In this study, we analyzed the potential of mitogen-activated protein kinase 3 (MAPK3) and mitogen-activated protein kinase 4 (MAPK4) proteins from Leishmania braziliensis to serve as antigen candidates for the serodiagnosis of human visceral and tegumentary leishmaniasis, as well as canine visceral disease. Moreover, we mapped linear B-cell epitopes in these proteins and selected those epitopes with sequences that were divergent in the corresponding orthologs in Homo sapiens, in Canis familiaris, and in Trypanosoma cruzi. We compared the performance of these peptides with the recombinant protein using ELISA. Both MAPK3 and MAPK4 recombinant proteins showed better specificity in the immunodiagnosis of human and canine leishmaniasis than soluble parasite antigens and the EIE-leishmaniose-visceral-canina-bio-manguinhos (EIE-LVC) kit. Furthermore, the performance of this serodiagnosis assay was improved using synthetic peptides corresponding to B-cell epitopes derived from both proteins. Springer-Verlag Berlin Heidelberg, 2014 Tegumentary and visceral human leishmaniasis nationallicence Canine visceral leishmaniasis nationallicence Immunodiagnosis nationallicence MAPK3 nationallicence MAPK4 nationallicence B-cell epitope mapping nationallicence Menezes-Souza Daniel Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil aut de Oliveira Mendes Tiago Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil aut de Araújo Leão Ana Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil aut de Souza Gomes Matheus Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Patos de Minas, Minas Gerais, Brazil aut Fujiwara Ricardo Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil aut Bartholomeu Daniella Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1323-1336 0175-7598 99:3<1323 2015 99 253 https://doi.org/10.1007/s00253-014-6168-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6168-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Menezes-Souza Daniel Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil aut NATIONALLICENCE 700 1- de Oliveira Mendes Tiago Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil aut NATIONALLICENCE 700 1- de Araújo Leão Ana Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil aut NATIONALLICENCE 700 1- de Souza Gomes Matheus Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Patos de Minas, Minas Gerais, Brazil aut NATIONALLICENCE 700 1- Fujiwara Ricardo Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil aut NATIONALLICENCE 700 1- Bartholomeu Daniella Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1323-1336 0175-7598 99:3<1323 2015 99 253