caa a22 4500 605501882 CHVBK 20210128100603.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-6359-2 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6359-2 The transcriptomic profile of Pseudozyma aphidis during production of mannosylerythritol lipids [Elektronische Daten] [Michael Günther, Christian Grumaz, Stefan Lorenz, Philip Stevens, Elena Lindemann, Thomas Hirth, Kai Sohn, Susanne Zibek, Steffen Rupp] The basidiomycetous fungus Pseudozyma aphidis is able to convert vegetable oils to abundant amounts of the biosurfactant mannosylerythritol lipid (MEL) with a unique product pattern of MEL-A, MEL-B, MEL-C, and MEL-D. To investigate the metabolism of MEL production, we analyzed the transcriptome of P. aphidis DSM 70725 under MEL-inducing and non-inducing conditions using deep sequencing. Following manual curation of the previously described in silico gene models based on RNA-Seq data, we were able to generate an experimentally verified gene annotation containing 6347 genes. Using this database, our expression analysis revealed that only four of the five cluster genes required for MEL synthesis were clearly induced by the presence of soybean oil. The acetyltransferase encoding gene PaGMAT1 was expressed on a much lower level, which may explain the secretion of MEL with different degrees of acetylation in P. aphidis. In parallel to MEL synthesis, microscopic observations showed morphological changes accompanied by expression of genes responsible for cell development, indicative of a coregulation between MEL synthesis and cell morphology. In addition a set of transcription factors was identified which may be responsible for regulation of MEL synthesis and cell development. The upregulation of genes required for nitrogen metabolism and other assimilation processes indicate additional metabolic pathways required under the MEL-inducing conditions used. We also searched for a conserved gene cluster for cellobiose lipids (CL) but only found seven genes with limited homology distributed over the genome. However, we detected characteristic TLC spots in fermentations using P. aphidis DSM 70725, indicative of CL secretion. Springer-Verlag Berlin Heidelberg, 2015 Transcriptome nationallicence Pseudozyma aphidis nationallicence Mannosylerythritol lipids nationallicence MEL gene cluster nationallicence Cellobiose lipids nationallicence Morphologic switch nationallicence Günther Michael Department of Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstr. 12, 70569, Stuttgart, Germany aut Grumaz Christian Department of Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstr. 12, 70569, Stuttgart, Germany aut Lorenz Stefan Department of Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstr. 12, 70569, Stuttgart, Germany aut Stevens Philip Institute of Interfacial Process Engineering and Plasma Technology, University of Stuttgart, Nobelstr. 12, 70569, Stuttgart, Germany aut Lindemann Elena Department of Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstr. 12, 70569, Stuttgart, Germany aut Hirth Thomas Department of Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstr. 12, 70569, Stuttgart, Germany aut Sohn Kai Department of Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstr. 12, 70569, Stuttgart, Germany aut Zibek Susanne Department of Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstr. 12, 70569, Stuttgart, Germany aut Rupp Steffen Department of Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstr. 12, 70569, Stuttgart, Germany aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1375-1388 0175-7598 99:3<1375 2015 99 253 https://doi.org/10.1007/s00253-014-6359-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6359-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Günther Michael Department of Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstr. 12, 70569, Stuttgart, Germany aut NATIONALLICENCE 700 1- Grumaz Christian Department of Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstr. 12, 70569, Stuttgart, Germany aut NATIONALLICENCE 700 1- Lorenz Stefan Department of Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstr. 12, 70569, Stuttgart, Germany aut NATIONALLICENCE 700 1- Stevens Philip Institute of Interfacial Process Engineering and Plasma Technology, University of Stuttgart, Nobelstr. 12, 70569, Stuttgart, Germany aut NATIONALLICENCE 700 1- Lindemann Elena Department of Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstr. 12, 70569, Stuttgart, Germany aut NATIONALLICENCE 700 1- Hirth Thomas Department of Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstr. 12, 70569, Stuttgart, Germany aut NATIONALLICENCE 700 1- Sohn Kai Department of Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstr. 12, 70569, Stuttgart, Germany aut NATIONALLICENCE 700 1- Zibek Susanne Department of Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstr. 12, 70569, Stuttgart, Germany aut NATIONALLICENCE 700 1- Rupp Steffen Department of Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstr. 12, 70569, Stuttgart, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1375-1388 0175-7598 99:3<1375 2015 99 253