Transcriptome-guided identification of SprA as a pleiotropic regulator in Streptomyces chattanoogensis
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6132-6 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6132-6 | ||
| 245 | 0 | 0 | |a Transcriptome-guided identification of SprA as a pleiotropic regulator in Streptomyces chattanoogensis |h [Elektronische Daten] |c [Zhen-Xing Zhou, Qing-Qing Xu, Qing-Ting Bu, Shui-Ping Liu, Pin Yu, Yong-Quan Li] |
| 520 | 3 | |a Quorum sensing molecular γ-butyrolactones (GBL) are widely distributed among the genus Streptomyces. Their cognate receptors have been demonstrated to control secondary metabolism and/or morphological differentiation. ScgA is responsible for the biosynthesis of GBL in Streptomyces chattanoogensis. According to the genome-wide transcriptome analysis of the ΔscgA mutant, we found that the expression of sprA, which encodes a GBL receptor homologue, was shown to be positively regulated by ScgA. Electrophoretic mobility shift assays and DNase I footprinting assays showed that SprA bound to two specific autoregulatory element (ARE) sequences located upstream of the sprA gene, indicating that its expression is self-regulated. SprA was involved in biosynthesis of GBL by repressing the expression of scgA. An Escherichia coli-based luciferase report system demonstrated that SprA directly repressed the expression of scgR, which encodes a GBL receptor. Like deletion of scgA, the disruption of sprA resulted in decreased production of the antibiotic natamycin in liquid culture and retarded morphological differentiation on solid agar. This work indicates that SprA acts as a pleiotropic regulator of both morphogenesis and the production of natamycin. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a GBL receptor |2 nationallicence | |
| 690 | 7 | |a Morphogenesis |2 nationallicence | |
| 690 | 7 | |a Secondary metabolism |2 nationallicence | |
| 690 | 7 | |a Streptomyces |2 nationallicence | |
| 700 | 1 | |a Zhou |D Zhen-Xing |u Institute of Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China |4 aut | |
| 700 | 1 | |a Xu |D Qing-Qing |u Institute of Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China |4 aut | |
| 700 | 1 | |a Bu |D Qing-Ting |u Institute of Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China |4 aut | |
| 700 | 1 | |a Liu |D Shui-Ping |u Institute of Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China |4 aut | |
| 700 | 1 | |a Yu |D Pin |u Institute of Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China |4 aut | |
| 700 | 1 | |a Li |D Yong-Quan |u Institute of Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/3(2015-02-01), 1287-1298 |x 0175-7598 |q 99:3<1287 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6132-6 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6132-6 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhou |D Zhen-Xing |u Institute of Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Xu |D Qing-Qing |u Institute of Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bu |D Qing-Ting |u Institute of Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Shui-Ping |u Institute of Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yu |D Pin |u Institute of Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Yong-Quan |u Institute of Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/3(2015-02-01), 1287-1298 |x 0175-7598 |q 99:3<1287 |1 2015 |2 99 |o 253 | ||