caa a22 4500 605501912 CHVBK 20210128100603.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-6132-6 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6132-6 Transcriptome-guided identification of SprA as a pleiotropic regulator in Streptomyces chattanoogensis [Elektronische Daten] [Zhen-Xing Zhou, Qing-Qing Xu, Qing-Ting Bu, Shui-Ping Liu, Pin Yu, Yong-Quan Li] Quorum sensing molecular γ-butyrolactones (GBL) are widely distributed among the genus Streptomyces. Their cognate receptors have been demonstrated to control secondary metabolism and/or morphological differentiation. ScgA is responsible for the biosynthesis of GBL in Streptomyces chattanoogensis. According to the genome-wide transcriptome analysis of the ΔscgA mutant, we found that the expression of sprA, which encodes a GBL receptor homologue, was shown to be positively regulated by ScgA. Electrophoretic mobility shift assays and DNase I footprinting assays showed that SprA bound to two specific autoregulatory element (ARE) sequences located upstream of the sprA gene, indicating that its expression is self-regulated. SprA was involved in biosynthesis of GBL by repressing the expression of scgA. An Escherichia coli-based luciferase report system demonstrated that SprA directly repressed the expression of scgR, which encodes a GBL receptor. Like deletion of scgA, the disruption of sprA resulted in decreased production of the antibiotic natamycin in liquid culture and retarded morphological differentiation on solid agar. This work indicates that SprA acts as a pleiotropic regulator of both morphogenesis and the production of natamycin. Springer-Verlag Berlin Heidelberg, 2014 GBL receptor nationallicence Morphogenesis nationallicence Secondary metabolism nationallicence Streptomyces nationallicence Zhou Zhen-Xing Institute of Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China aut Xu Qing-Qing Institute of Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China aut Bu Qing-Ting Institute of Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China aut Liu Shui-Ping Institute of Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China aut Yu Pin Institute of Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China aut Li Yong-Quan Institute of Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1287-1298 0175-7598 99:3<1287 2015 99 253 https://doi.org/10.1007/s00253-014-6132-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6132-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Zhen-Xing Institute of Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Xu Qing-Qing Institute of Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Bu Qing-Ting Institute of Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Liu Shui-Ping Institute of Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Yu Pin Institute of Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Li Yong-Quan Institute of Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1287-1298 0175-7598 99:3<1287 2015 99 253