Promiscuous activity of ( S , S )-butanediol dehydrogenase is responsible for glycerol production from 1,3-dihydroxyacetone in Corynebacterium glutamicum under oxygen-deprived conditions
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6170-0 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6170-0 | ||
| 245 | 0 | 0 | |a Promiscuous activity of ( S , S )-butanediol dehydrogenase is responsible for glycerol production from 1,3-dihydroxyacetone in Corynebacterium glutamicum under oxygen-deprived conditions |h [Elektronische Daten] |c [Toru Jojima, Takafumi Igari, Yasuhiro Moteki, Masako Suda, Hideaki Yukawa, Masayuki Inui] |
| 520 | 3 | |a Corynebacterium glutamicum can consume glucose to excrete glycerol under oxygen deprivation. Although glycerol synthesis from 1,3-dihydroxyacetone (DHA) has been speculated, no direct evidence has yet been provided in C. glutamicum. Enzymatic and genetic investigations here indicate that the glycerol is largely produced from DHA and, unexpectedly, the reaction is catalyzed by (S,S)-butanediol dehydrogenase (ButA) that inherently catalyzes the interconversion between S-acetoin and (S,S)-2,3-butanediol. Consequently, the following pathway for glycerol biosynthesis in the bacterium emerges: dihydroxyacetone phosphate is dephosphorylated by HdpA to DHA, which is subsequently reduced to glycerol by ButA. This study emphasizes the importance of promiscuous activity of the enzyme in vivo. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Corynebacterium glutamicum |2 nationallicence | |
| 690 | 7 | |a Glycerol |2 nationallicence | |
| 690 | 7 | |a 2,3-Butanediol dehydrogenase |2 nationallicence | |
| 690 | 7 | |a ButA |2 nationallicence | |
| 690 | 7 | |a Enzyme promiscuity |2 nationallicence | |
| 700 | 1 | |a Jojima |D Toru |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | |
| 700 | 1 | |a Igari |D Takafumi |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | |
| 700 | 1 | |a Moteki |D Yasuhiro |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | |
| 700 | 1 | |a Suda |D Masako |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | |
| 700 | 1 | |a Yukawa |D Hideaki |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | |
| 700 | 1 | |a Inui |D Masayuki |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/3(2015-02-01), 1427-1433 |x 0175-7598 |q 99:3<1427 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6170-0 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6170-0 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Jojima |D Toru |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Igari |D Takafumi |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Moteki |D Yasuhiro |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Suda |D Masako |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yukawa |D Hideaki |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Inui |D Masayuki |u Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/3(2015-02-01), 1427-1433 |x 0175-7598 |q 99:3<1427 |1 2015 |2 99 |o 253 | ||