caa a22 4500 605501947 CHVBK 20210128100603.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-6170-0 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6170-0 Promiscuous activity of ( S , S )-butanediol dehydrogenase is responsible for glycerol production from 1,3-dihydroxyacetone in Corynebacterium glutamicum under oxygen-deprived conditions [Elektronische Daten] [Toru Jojima, Takafumi Igari, Yasuhiro Moteki, Masako Suda, Hideaki Yukawa, Masayuki Inui] Corynebacterium glutamicum can consume glucose to excrete glycerol under oxygen deprivation. Although glycerol synthesis from 1,3-dihydroxyacetone (DHA) has been speculated, no direct evidence has yet been provided in C. glutamicum. Enzymatic and genetic investigations here indicate that the glycerol is largely produced from DHA and, unexpectedly, the reaction is catalyzed by (S,S)-butanediol dehydrogenase (ButA) that inherently catalyzes the interconversion between S-acetoin and (S,S)-2,3-butanediol. Consequently, the following pathway for glycerol biosynthesis in the bacterium emerges: dihydroxyacetone phosphate is dephosphorylated by HdpA to DHA, which is subsequently reduced to glycerol by ButA. This study emphasizes the importance of promiscuous activity of the enzyme in vivo. Springer-Verlag Berlin Heidelberg, 2014 Corynebacterium glutamicum nationallicence Glycerol nationallicence 2,3-Butanediol dehydrogenase nationallicence ButA nationallicence Enzyme promiscuity nationallicence Jojima Toru Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut Igari Takafumi Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut Moteki Yasuhiro Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut Suda Masako Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut Yukawa Hideaki Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut Inui Masayuki Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1427-1433 0175-7598 99:3<1427 2015 99 253 https://doi.org/10.1007/s00253-014-6170-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6170-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jojima Toru Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut NATIONALLICENCE 700 1- Igari Takafumi Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut NATIONALLICENCE 700 1- Moteki Yasuhiro Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut NATIONALLICENCE 700 1- Suda Masako Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut NATIONALLICENCE 700 1- Yukawa Hideaki Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut NATIONALLICENCE 700 1- Inui Masayuki Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1427-1433 0175-7598 99:3<1427 2015 99 253