caa a22 4500 605501955 CHVBK 20210128100603.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-6042-7 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6042-7 Crude oil degradation by bacterial consortia under four different redox and temperature conditions [Elektronische Daten] [Shunzi Xiong, Xia Li, Jianfa Chen, Liping Zhao, Hui Zhang, Xiaojun Zhang] There is emerging interest in the anaerobic degradation of crude oil. However, there is limited knowledge about the geochemical effects and microbiological activities for it. A mixture of anaerobic sludge and the production water from an oil well was used as an inoculum to construct four consortia, which were incubated under sulfate-reducing or methanogenic conditions at either mesophilic or thermophilic temperatures. Significant degradation of saturated and aromatic hydrocarbons and the changing quantities of some marker compounds, such as pristane, phytane, hopane and norhopane, and their relative quantities, suggested the activity of microorganisms in the consortia. Notably, the redox conditions and temperature strongly affected the diversity and structure of the enriched microbial communities and the oil degradation. Although some specific biomarker showed larger change under methanogenic condition, the degradation efficiencies for total aromatic and saturated hydrocarbon were higher under sulfate-reducing condition. After the 540-day incubation, bacteria of unknown classifications were dominant in the thermophilic methanogenic consortia, whereas Clostridium dominated the mesophilic methanogenic consortia. With the exception of the dominant phylotypes that were shared with the methanogenic consortia, the sulfate-reducing consortia were predominantly composed of Thermotogae, Deltaproteobacteria, Spirochaeta, and Synergistetes phyla. In conclusion, results in this study demonstrated that the different groups of degraders were responsible for degradation in the four constructed crude oil degrading consortia and consequently led to the existence of different amount of marker compounds under these distinct conditions. There might be distinct metabolic mechanism for degrading crude oil under sulfate-reducing and methanogenic conditions. Springer-Verlag Berlin Heidelberg, 2014 Crude oil nationallicence Norhopane nationallicence Methanogenic nationallicence Sulfate-reducing nationallicence Bacterial degradation nationallicence Bacterial community nationallicence Xiong Shunzi State Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut Li Xia Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China aut Chen Jianfa State Key Laboratory of Petroleum Resources and Prospecting, China University of Petroleum, 102249, Beijing, China aut Zhao Liping State Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut Zhang Hui Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China aut Zhang Xiaojun State Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1451-1461 0175-7598 99:3<1451 2015 99 253 https://doi.org/10.1007/s00253-014-6042-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6042-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xiong Shunzi State Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Li Xia Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China aut NATIONALLICENCE 700 1- Chen Jianfa State Key Laboratory of Petroleum Resources and Prospecting, China University of Petroleum, 102249, Beijing, China aut NATIONALLICENCE 700 1- Zhao Liping State Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Hui Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China aut NATIONALLICENCE 700 1- Zhang Xiaojun State Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1451-1461 0175-7598 99:3<1451 2015 99 253