caa a22 4500 605501963 CHVBK 20210128100603.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-6054-3 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6054-3 The biosurfactant viscosin transiently stimulates n-hexadecane mineralization by a bacterial consortium [Elektronische Daten] [Frederik Bak, Lise Bonnichsen, Niels Jørgensen, Mette Nicolaisen, Ole Nybroe] Pseudomonas produces powerful lipopeptide biosurfactants including viscosin, massetolide A, putisolvin, and amphisin, but their ability to stimulate alkane mineralization and their utility for bioremediation have received limited attention. The four Pseudomonas lipopeptides yielded emulsification indices on hexadecane of 20-31% at 90mg/l, which is comparable to values for the synthetic surfactant Tween 80. Viscosin was the optimal emulsifier and significantly stimulated n-hexadecane mineralization by diesel-degrading bacterial consortia but exclusively during the first 2days of batch culture experiments. Growth of the consortia, as determined by OD600 measurements and quantification of the alkB marker gene for alkane degradation, was arrested after the first day of the experiment. In contrast, the control consortia continued to grow and reached higher OD600 values and higher alkB copy numbers during the next days. Due to the short-lived stimulation of n-hexadecane mineralization, the stability of viscosin was analyzed, and it was observed that added viscosin was degraded by the bacterial consortium during the first 2days. Hence, viscosin has a potential as stimulator of alkane degradation, but its utility in bioremediation may be limited by its rapid degradation and growth-inhibiting properties. The Author(s), 2014 Alkanes nationallicence Biodegradation nationallicence Lipopeptides nationallicence Pseudomonas nationallicence Bak Frederik Section of Genetics and Microbiology, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Copenhagen, Denmark aut Bonnichsen Lise Section of Genetics and Microbiology, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Copenhagen, Denmark aut Jørgensen Niels Section of Genetics and Microbiology, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Copenhagen, Denmark aut Nicolaisen Mette Section of Genetics and Microbiology, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Copenhagen, Denmark aut Nybroe Ole Section of Genetics and Microbiology, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Copenhagen, Denmark aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1475-1483 0175-7598 99:3<1475 2015 99 253 https://doi.org/10.1007/s00253-014-6054-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6054-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bak Frederik Section of Genetics and Microbiology, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Bonnichsen Lise Section of Genetics and Microbiology, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Jørgensen Niels Section of Genetics and Microbiology, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Nicolaisen Mette Section of Genetics and Microbiology, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Nybroe Ole Section of Genetics and Microbiology, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Copenhagen, Denmark aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1475-1483 0175-7598 99:3<1475 2015 99 253