Immobilised whole-cell recombinant monoamine oxidase biocatalysis
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| 024 | 7 | 0 | |a 10.1007/s00253-014-5983-1 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-5983-1 | ||
| 245 | 0 | 0 | |a Immobilised whole-cell recombinant monoamine oxidase biocatalysis |h [Elektronische Daten] |c [Petra Zajkoska, Michal Rosenberg, Rachel Heath, Kirk Malone, Radek Stloukal, Nicholas Turner, Martin Rebroš] |
| 520 | 3 | |a This work demonstrates the first example of the immobilisation of MAO-N whole cells to produce a biocatalyst that remained suitable for repetitive use after 11months of storage and stable up to 15months after immobilisation. The production of Escherichia coli expressing recombinant MAO-N was scaled up to bioreactors under regulated, previously optimised conditions (10% DO, pH7), and the amount of biomass was almost doubled compared to flask cultivation. Subsequently, pilot immobilisation of the whole-cell biocatalyst using LentiKats® technology was performed. The amount of the immobilised biomass was optimised and the process was scaled up to a production level by immobilising 15g of dry cell weight per litre of polyvinyl alcohol to produce 3kg of whole-cell ready-to-use biocatalyst. The immobilised biocatalyst retained its initial activity over six consecutive biotransformations of the secondary amine model compound 3-azabicylo [3,3,0]octane, a building block of the hepatitis C drug telaprevir. Consecutive cultivation cycles in growth conditions not only increased the initial specific activity of biocatalyst produced on the industrial plant by more than 30%, but also significantly increased the rate of the biotransformation compared to the non-propagated biocatalyst. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Immobilisation |2 nationallicence | |
| 690 | 7 | |a E. coli |2 nationallicence | |
| 690 | 7 | |a MAO-N |2 nationallicence | |
| 690 | 7 | |a Biotransformation |2 nationallicence | |
| 690 | 7 | |a Amine |2 nationallicence | |
| 700 | 1 | |a Zajkoska |D Petra |u Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia |4 aut | |
| 700 | 1 | |a Rosenberg |D Michal |u Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia |4 aut | |
| 700 | 1 | |a Heath |D Rachel |u School of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, M1 7DN, Manchester, UK |4 aut | |
| 700 | 1 | |a Malone |D Kirk |u School of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, M1 7DN, Manchester, UK |4 aut | |
| 700 | 1 | |a Stloukal |D Radek |u LentiKats a.s, Evropská 846/176a, 160 00, Prague 6, Czech Republic |4 aut | |
| 700 | 1 | |a Turner |D Nicholas |u School of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, M1 7DN, Manchester, UK |4 aut | |
| 700 | 1 | |a Rebroš |D Martin |u Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/3(2015-02-01), 1229-1236 |x 0175-7598 |q 99:3<1229 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-5983-1 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-5983-1 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zajkoska |D Petra |u Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Rosenberg |D Michal |u Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Heath |D Rachel |u School of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, M1 7DN, Manchester, UK |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Malone |D Kirk |u School of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, M1 7DN, Manchester, UK |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Stloukal |D Radek |u LentiKats a.s, Evropská 846/176a, 160 00, Prague 6, Czech Republic |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Turner |D Nicholas |u School of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, M1 7DN, Manchester, UK |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Rebroš |D Martin |u Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/3(2015-02-01), 1229-1236 |x 0175-7598 |q 99:3<1229 |1 2015 |2 99 |o 253 | ||