caa a22 4500 605501971 CHVBK 20210128100603.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-5983-1 doi (NATIONALLICENCE)springer-10.1007/s00253-014-5983-1 Immobilised whole-cell recombinant monoamine oxidase biocatalysis [Elektronische Daten] [Petra Zajkoska, Michal Rosenberg, Rachel Heath, Kirk Malone, Radek Stloukal, Nicholas Turner, Martin Rebroš] This work demonstrates the first example of the immobilisation of MAO-N whole cells to produce a biocatalyst that remained suitable for repetitive use after 11months of storage and stable up to 15months after immobilisation. The production of Escherichia coli expressing recombinant MAO-N was scaled up to bioreactors under regulated, previously optimised conditions (10% DO, pH7), and the amount of biomass was almost doubled compared to flask cultivation. Subsequently, pilot immobilisation of the whole-cell biocatalyst using LentiKats® technology was performed. The amount of the immobilised biomass was optimised and the process was scaled up to a production level by immobilising 15g of dry cell weight per litre of polyvinyl alcohol to produce 3kg of whole-cell ready-to-use biocatalyst. The immobilised biocatalyst retained its initial activity over six consecutive biotransformations of the secondary amine model compound 3-azabicylo [3,3,0]octane, a building block of the hepatitis C drug telaprevir. Consecutive cultivation cycles in growth conditions not only increased the initial specific activity of biocatalyst produced on the industrial plant by more than 30%, but also significantly increased the rate of the biotransformation compared to the non-propagated biocatalyst. Springer-Verlag Berlin Heidelberg, 2014 Immobilisation nationallicence E. coli nationallicence MAO-N nationallicence Biotransformation nationallicence Amine nationallicence Zajkoska Petra Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia aut Rosenberg Michal Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia aut Heath Rachel School of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, M1 7DN, Manchester, UK aut Malone Kirk School of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, M1 7DN, Manchester, UK aut Stloukal Radek LentiKats a.s, Evropská 846/176a, 160 00, Prague 6, Czech Republic aut Turner Nicholas School of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, M1 7DN, Manchester, UK aut Rebroš Martin Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1229-1236 0175-7598 99:3<1229 2015 99 253 https://doi.org/10.1007/s00253-014-5983-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-5983-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zajkoska Petra Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia aut NATIONALLICENCE 700 1- Rosenberg Michal Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia aut NATIONALLICENCE 700 1- Heath Rachel School of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, M1 7DN, Manchester, UK aut NATIONALLICENCE 700 1- Malone Kirk School of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, M1 7DN, Manchester, UK aut NATIONALLICENCE 700 1- Stloukal Radek LentiKats a.s, Evropská 846/176a, 160 00, Prague 6, Czech Republic aut NATIONALLICENCE 700 1- Turner Nicholas School of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, M1 7DN, Manchester, UK aut NATIONALLICENCE 700 1- Rebroš Martin Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1229-1236 0175-7598 99:3<1229 2015 99 253