caa a22 4500 60550198X CHVBK 20210128100603.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-6063-2 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6063-2 Bacterial community analysis by PCR-DGGE and 454-pyrosequencing of horizontal subsurface flow constructed wetlands with front aeration [Elektronische Daten] [Fei Zhong, Juan Wu, Yanran Dai, Lihua Yang, Zhaohui Zhang, Shuiping Cheng, Qiong Zhang] Horizontal subsurface flow constructed wetlands (HSSF CWs) with and without redox manipulation by front aeration were operated to treat mechanically pretreated wastewater from a nearby wastewater treatment plant. Polymerase chain reaction-denaturing gradient gel electrophoresis and 454-pyrosequencing were used to characterize the shifts in bacterial community diversity and composition in response to front aeration in the HSSF CWs. Both techniques revealed similar bacterial diversity between the HSSF CWs with (ACW) and without front aeration (NACW). Differences in microbial functional groups between the ACW and the NACW substrate samples were identified with 454-pyrosequencing. Nitrite-oxidizing bacteria (Nitrospira) and ammonia-oxidizing bacteria (Nitrosomonas) had much higher abundances in the ACW, whereas more sequences related to sulfate-reducing bacteria and anaerobic sulfur-oxidizing bacteria (genera Sulfuricella, Sulfuritalea, and Sulfuricurvum) were detected in the NACW. Removal efficiencies for NH4 +-N, PO4 3−-P and chemical oxygen demand in the ACW were 48.7 ± 15.5, 70.2 ± 13.5, and 82.0 ± 6.4%, respectively, whereas the removal efficiencies for these parameters in the NACW were 10.3 ± 14.0, 53.1 ± 18.9, and 68.8 ± 10.7%, respectively. In the ACW, the stimulation of nitrification via front aeration supplied more NO2 −-N and NO3 −-N to the subsequent denitrification process than in the NACW, resulting in higher total inorganic nitrogen removal efficiency. The differences in treatment efficiencies between the ACW and the NACW could be partially explained by the different bacterial community compositions in the two CWs. Springer-Verlag Berlin Heidelberg, 2014 Constructed wetland nationallicence Front aeration nationallicence Bacterial community nationallicence DGGE nationallicence 454-Pyrosequencing nationallicence Zhong Fei State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, 200092, Shanghai, People's Republic of China aut Wu Juan State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, 200092, Shanghai, People's Republic of China aut Dai Yanran State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, 200092, Shanghai, People's Republic of China aut Yang Lihua State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, 200092, Shanghai, People's Republic of China aut Zhang Zhaohui Marine Fisheries Research Institute of Jiangsu Province, 226007, Nantong, People's Republic of China aut Cheng Shuiping State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, 200092, Shanghai, People's Republic of China aut Zhang Qiong Department of Civil and Environmental Engineering, University of South Florida, 4202 E. Fowler Ave., ENB 118, Tampa, FL, USA aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1499-1512 0175-7598 99:3<1499 2015 99 253 https://doi.org/10.1007/s00253-014-6063-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6063-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhong Fei State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, 200092, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Wu Juan State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, 200092, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Dai Yanran State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, 200092, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Yang Lihua State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, 200092, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Zhaohui Marine Fisheries Research Institute of Jiangsu Province, 226007, Nantong, People's Republic of China aut NATIONALLICENCE 700 1- Cheng Shuiping State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, 200092, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Qiong Department of Civil and Environmental Engineering, University of South Florida, 4202 E. Fowler Ave., ENB 118, Tampa, FL, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/3(2015-02-01), 1499-1512 0175-7598 99:3<1499 2015 99 253