caa a22 4500 605501998 CHVBK 20210128100603.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00253-015-6536-y doi (NATIONALLICENCE)springer-10.1007/s00253-015-6536-y 16S rRNA gene high-throughput sequencing data mining of microbial diversity and interactions [Elektronische Daten] [Feng Ju, Tong Zhang] The ubiquitous occurrence of microorganisms gives rise to continuous public concerns regarding their pathogenicity and threats to human environment, as well as potential engineering benefits in biotechnology. The development and wide application of environmental biotechnology, for example in bioenergy production, wastewater treatment, bioremediation, and drinking water disinfection, have been bringing us with both environmental and economic benefits. Strikingly, extensive applications of microscopic and molecular techniques since 1990s have allowed engineers to peep into the microbiology in "black box” of engineered microbial communities in biotechnological processes, providing guidelines for process design and optimization. Recently, revolutionary advances in DNA sequencing technologies and rapidly decreasing costs are altering conventional ways of microbiology and ecology research, as it launches an era of next-generation sequencing (NGS). The principal research burdens are now transforming from traditional labor-intensive wet-lab experiments to dealing with analysis of huge and informative NGS data, which is computationally expensive and bioinformatically challenging. This study discusses state-of-the-art bioinformatics and statistical analyses of 16S ribosomal RNA (rRNA) gene high-throughput sequencing (HTS) data from prevalent NGS platforms to promote its applications in exploring microbial diversity of functional and pathogenic microorganisms, as well as their interactions in biotechnological processes. Springer-Verlag Berlin Heidelberg, 2015 Next-generation sequencing nationallicence 16S rRNA gene nationallicence Bioinformatics and statistics nationallicence Network analysis nationallicence Microbiology nationallicence Biotechnology nationallicence Ju Feng Environmental Biotechnology Lab, The University of Hong Kong, Pokfulam Road, Hong Kong, China aut Zhang Tong Environmental Biotechnology Lab, The University of Hong Kong, Pokfulam Road, Hong Kong, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4119-4129 0175-7598 99:10<4119 2015 99 253 https://doi.org/10.1007/s00253-015-6536-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6536-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ju Feng Environmental Biotechnology Lab, The University of Hong Kong, Pokfulam Road, Hong Kong, China aut NATIONALLICENCE 700 1- Zhang Tong Environmental Biotechnology Lab, The University of Hong Kong, Pokfulam Road, Hong Kong, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4119-4129 0175-7598 99:10<4119 2015 99 253