caa a22 4500 605502005 CHVBK 20210128100603.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00253-014-6356-5 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6356-5 Microbial diversity observed during hemp retting [Elektronische Daten] [Alexandra Ribeiro, Philippe Pochart, Arnaud Day, Sarah Mennuni, Pierre Bono, Jean-Luc Baret, Jean-Louis Spadoni, Irène Mangin] Historically used in textile and paper industry, hemp fibres have started to find new applications in composite materials with important economic and ecological advantages. However, their applications are limited since manufacturers have some difficulties to standardise fabrication processes. This study is a first step before selection and isolation of strains that could later be used to optimise microbial retting efficiency and hence fibre quality. We studied six samples harvested on different ground types, at different dates and with different retting durations on field to obtain an exhaustive representation of the process. After DNA extraction, total bacteria and fungi associated with stems during retting were specifically quantified using real-time PCR. Then, using sequence analysis of randomly cloned 16S and 18S ribosomal RNA (rRNA) genes, a phylogenetic characterisation of the dominant microorganisms was carried out. Quantitatively, we showed that there were 8.1-9.5 log10 16S rRNA gene copies per gram of hemp straw for bacteria and 8.6-9.6 log10 18S rRNA gene copies per gram for fungi. Qualitatively, we noticed a higher bacterial diversity in comparison to fungi. This work showed that in the different samples, the same species were present but in significantly different proportions according to ground type, harvest dates and retting durations on field. The most frequent bacterial sequences were affiliated to species Escherichia coli, Pantoea agglomerans, Pseudomonas rhizosphaerae, Rhodobacter sp., Pseudomonas fulva, Rhizobium huautlense and Massilia timonae, whereas fungal sequences were principally related to the genera Cladosporium and Cryptococcus. Springer-Verlag Berlin Heidelberg, 2015 Hemp retting nationallicence Microbiota nationallicence Molecular inventory nationallicence qPCR nationallicence Ribeiro Alexandra Microbial Ecology Laboratory, Conservatoire National des Arts et Métiers, 2 Rue Conté, 75003, Paris, France aut Pochart Philippe Microbial Ecology Laboratory, Conservatoire National des Arts et Métiers, 2 Rue Conté, 75003, Paris, France aut Day Arnaud Fibres Recherche Développement®, Technopole de l'Aube en Champagne, 10450, Bréviandes, France aut Mennuni Sarah Fibres Recherche Développement®, Technopole de l'Aube en Champagne, 10450, Bréviandes, France aut Bono Pierre Fibres Recherche Développement®, Technopole de l'Aube en Champagne, 10450, Bréviandes, France aut Baret Jean-Luc Laboratoire Industries agroalimentaires, Conservatoire National des Arts et Métiers, 292 Rue St Martin, 75003, Paris, France aut Spadoni Jean-Louis Laboratoire Génomique, Bioinformatique et Applications, Conservatoire National des Arts et Métiers, 2 Rue Conté, 75003, Paris, France aut Mangin Irène Microbial Ecology Laboratory, Conservatoire National des Arts et Métiers, 2 Rue Conté, 75003, Paris, France aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4471-4484 0175-7598 99:10<4471 2015 99 253 https://doi.org/10.1007/s00253-014-6356-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6356-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ribeiro Alexandra Microbial Ecology Laboratory, Conservatoire National des Arts et Métiers, 2 Rue Conté, 75003, Paris, France aut NATIONALLICENCE 700 1- Pochart Philippe Microbial Ecology Laboratory, Conservatoire National des Arts et Métiers, 2 Rue Conté, 75003, Paris, France aut NATIONALLICENCE 700 1- Day Arnaud Fibres Recherche Développement®, Technopole de l'Aube en Champagne, 10450, Bréviandes, France aut NATIONALLICENCE 700 1- Mennuni Sarah Fibres Recherche Développement®, Technopole de l'Aube en Champagne, 10450, Bréviandes, France aut NATIONALLICENCE 700 1- Bono Pierre Fibres Recherche Développement®, Technopole de l'Aube en Champagne, 10450, Bréviandes, France aut NATIONALLICENCE 700 1- Baret Jean-Luc Laboratoire Industries agroalimentaires, Conservatoire National des Arts et Métiers, 292 Rue St Martin, 75003, Paris, France aut NATIONALLICENCE 700 1- Spadoni Jean-Louis Laboratoire Génomique, Bioinformatique et Applications, Conservatoire National des Arts et Métiers, 2 Rue Conté, 75003, Paris, France aut NATIONALLICENCE 700 1- Mangin Irène Microbial Ecology Laboratory, Conservatoire National des Arts et Métiers, 2 Rue Conté, 75003, Paris, France aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4471-4484 0175-7598 99:10<4471 2015 99 253