caa a22 4500 605502021 CHVBK 20210128100603.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00253-015-6438-z doi (NATIONALLICENCE)springer-10.1007/s00253-015-6438-z A chip-based assay for botulinum neurotoxin A activity in pharmaceutical preparations [Elektronische Daten] [Christian Lévêque, Géraldine Ferracci, Yves Maulet, Chloé Grand-Masson, Michael Seagar, Oussama El Far] The production of botulinum neurotoxin A (BoNT/A) for therapeutic and cosmetic applications requires precise determination of batch potency, and the enzymatic activity of BoNT/A light chain is a crucial index that can be measured in vitro. We previously established a SNAP-25 chip-based assay using surface plasmon resonance (SPR) that is more sensitive than the standard mouse bioassay for the quantification of BoNT/A activity. We have now adapted this procedure for pharmaceutical preparations. The optimized SPR assay allowed multiple measurements on a single chip, including the kinetics of substrate cleavage. The activity of five different batches of a pharmaceutical BoNT/A preparation was determined in a blind study by SPR and found to be in agreement with data from the in vivo mouse lethality assay. Biosensor detection of specific proteolytic products has the potential to accurately monitor the activity of pharmaceutical BoNT/A preparations, and a single chip can be used to assay more than 100 samples. Springer-Verlag Berlin Heidelberg, 2015 Botulinum neurotoxin nationallicence Surface plasmon resonance nationallicence Toxin sensor nationallicence On-chip enzyme assay nationallicence Lévêque Christian INSERM, UMR_S 1072, 13015, Marseille, France aut Ferracci Géraldine CNRS, UMR 7286, Plate-Forme de Recherche en Neurosciences PFRN, 13015, Marseille, France aut Maulet Yves INSERM, UMR_S 1072, 13015, Marseille, France aut Grand-Masson Chloé CNRS, UMR 7286, Plate-Forme de Recherche en Neurosciences PFRN, 13015, Marseille, France aut Seagar Michael INSERM, UMR_S 1072, 13015, Marseille, France aut El Far Oussama INSERM, UMR_S 1072, 13015, Marseille, France aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4355-4360 0175-7598 99:10<4355 2015 99 253 https://doi.org/10.1007/s00253-015-6438-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6438-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lévêque Christian INSERM, UMR_S 1072, 13015, Marseille, France aut NATIONALLICENCE 700 1- Ferracci Géraldine CNRS, UMR 7286, Plate-Forme de Recherche en Neurosciences PFRN, 13015, Marseille, France aut NATIONALLICENCE 700 1- Maulet Yves INSERM, UMR_S 1072, 13015, Marseille, France aut NATIONALLICENCE 700 1- Grand-Masson Chloé CNRS, UMR 7286, Plate-Forme de Recherche en Neurosciences PFRN, 13015, Marseille, France aut NATIONALLICENCE 700 1- Seagar Michael INSERM, UMR_S 1072, 13015, Marseille, France aut NATIONALLICENCE 700 1- El Far Oussama INSERM, UMR_S 1072, 13015, Marseille, France aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4355-4360 0175-7598 99:10<4355 2015 99 253