caa a22 4500 60550203X CHVBK 20210128100603.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00253-015-6573-6 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6573-6 Simple and specific colorimetric detection of Staphylococcus using its volatile 2-[3-acetoxy-4,4,14-trimethylandrost-8-en-17-yl] propanoic acid in the liquid phase and head space of cultures [Elektronische Daten] [Raju Saranya, Raju Aarthi, Krishnan Sankaran] Spread of drug-resistant Staphylococcus spp. into communities pose danger demanding effective non-invasive and non-destructive tools for its early detection and surveillance. Characteristic volatile organic compounds (VOCs) produced by bacteria offer new diagnostic targets and novel approaches not exploited so far in infectious disease diagnostics. Our search for such characteristic VOC for Staphylococcus spp. led to the depiction of 2-[3-acetoxy-4,4,14-trimethylandrost-8-en-17-yl] propanoic acid (ATMAP), a moderately volatile compound detected both in the culture and headspace when the organism was grown in tryptone soya broth (TSB) medium. A simple and inexpensive colorimetric method (colour change from yellow to orange) using methyl red as the pH indicator provided an absolutely specific way for identifying Staphylococcus spp., The assay performed in liquid cultures (7-h growth in TSB) as well as in the headspace of plate cultures (grown for 10h on TSA) was optimised in a 96-well plate and 12-well plate formats, respectively, employing a set of positive and negative strains. Only Staphylococcus spp. showed the distinct colour change from yellow to orange due to the production of the above VOC while in the case of other organisms, the reagent remained yellow. The method validated using known clinical and environmental strains (56 including Staphylococcus, Proteus, Pseudomonas, Klebsiella, Bacillus, Shigella and Escherichia coli) was found to be highly efficient showing 100% specificity and sensitivity. Such simple methods of bacterial pathogen identification are expected to form the next generation tools for the control of infectious diseases through early detection and surveillance of causative agents. Springer-Verlag Berlin Heidelberg, 2015 Staphylococcus nationallicence Colorimetric nationallicence Methyl red nationallicence Surveillance nationallicence Nosocomial infection nationallicence Saranya Raju Centre for Biotechnology, Anna University, Sardar Patel road, 600 025, Guindy, Chennai, India aut Aarthi Raju Centre for Biotechnology, Anna University, Sardar Patel road, 600 025, Guindy, Chennai, India aut Sankaran Krishnan Centre for Biotechnology, Anna University, Sardar Patel road, 600 025, Guindy, Chennai, India aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4423-4433 0175-7598 99:10<4423 2015 99 253 https://doi.org/10.1007/s00253-015-6573-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6573-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saranya Raju Centre for Biotechnology, Anna University, Sardar Patel road, 600 025, Guindy, Chennai, India aut NATIONALLICENCE 700 1- Aarthi Raju Centre for Biotechnology, Anna University, Sardar Patel road, 600 025, Guindy, Chennai, India aut NATIONALLICENCE 700 1- Sankaran Krishnan Centre for Biotechnology, Anna University, Sardar Patel road, 600 025, Guindy, Chennai, India aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4423-4433 0175-7598 99:10<4423 2015 99 253