caa a22 4500 605502072 CHVBK 20210128100603.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00253-015-6386-7 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6386-7 Effects of simulated microgravity and spaceflight on morphological differentiation and secondary metabolism of Streptomyces coelicolor A3(2) [Elektronische Daten] [Bing Huang, Ning Liu, Xiaoying Rong, Jisheng Ruan, Ying Huang] As well-known antibiotic-producing and filamentous bacteria, streptomycetes can be an ideal model to study the effects of microgravity on microbial development and antibiotic production. In this study, the model organism Streptomyces coelicolor A3(2) was exposed to simulated microgravity (SMG) on a rotating clinostat and microgravity (μg) on the Shenzhou-8 spacecraft. The strain exhibited some similar responses under both conditions. Compared with the controls, its life cycle in agar medium was shortened relatively, and the sporulation process was accelerated with higher accumulation of the gray spore pigment; the liquid cultures yielded more cell biomass, coupled with thicker, more fragmented, and well-dispersed hyphae of the μg spaceflight samples. Global transcriptional analysis verified that most of the differentially expressed genes involved in morphological differentiation of S. coelicolor were upregulated during days 4-6 under SMG conditions, notably the whi genes (whiD, sigF, and whiE). Production of actinorhodin (ACT) in agar cultures decreased under both conditions while undecylprodigiosin (RED) was produced earlier, which were consistent with the transcriptional levels of act and red gene clusters. Meanwhile, expression of the gene clusters for calcium-dependent antibiotic (CDA), methylenomycin (MMY), and a cryptic polyketide (CPK) was unchanged, downregulated, and upregulated, respectively, the latter of which might contribute to the enhanced activity of S. coelicolor against Bacillus subtilis under microgravity. Our study provides new insights into the morphological and secondary metabolic responses of streptomycetes to microgravity. Springer-Verlag Berlin Heidelberg, 2015 Streptomyces coelicolor nationallicence Spaceflight nationallicence Simulated microgravity nationallicence Morphological differentiation nationallicence Secondary metabolism nationallicence Shenzhou-8 space mission nationallicence Huang Bing State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, NO. 1 Beichen West Road, 100101, Beijing, Chaoyang District, China aut Liu Ning State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, NO. 1 Beichen West Road, 100101, Beijing, Chaoyang District, China aut Rong Xiaoying State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, NO. 1 Beichen West Road, 100101, Beijing, Chaoyang District, China aut Ruan Jisheng State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, NO. 1 Beichen West Road, 100101, Beijing, Chaoyang District, China aut Huang Ying State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, NO. 1 Beichen West Road, 100101, Beijing, Chaoyang District, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4409-4422 0175-7598 99:10<4409 2015 99 253 https://doi.org/10.1007/s00253-015-6386-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6386-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huang Bing State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, NO. 1 Beichen West Road, 100101, Beijing, Chaoyang District, China aut NATIONALLICENCE 700 1- Liu Ning State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, NO. 1 Beichen West Road, 100101, Beijing, Chaoyang District, China aut NATIONALLICENCE 700 1- Rong Xiaoying State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, NO. 1 Beichen West Road, 100101, Beijing, Chaoyang District, China aut NATIONALLICENCE 700 1- Ruan Jisheng State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, NO. 1 Beichen West Road, 100101, Beijing, Chaoyang District, China aut NATIONALLICENCE 700 1- Huang Ying State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, NO. 1 Beichen West Road, 100101, Beijing, Chaoyang District, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4409-4422 0175-7598 99:10<4409 2015 99 253