Dirhamnose-lipid production by recombinant nonpathogenic bacterium Pseudomonas chlororaphis
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 605502129 | ||
| 003 | CHVBK | ||
| 005 | 20210128100604.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 210128e20150501xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6433-4 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6433-4 | ||
| 245 | 0 | 0 | |a Dirhamnose-lipid production by recombinant nonpathogenic bacterium Pseudomonas chlororaphis |h [Elektronische Daten] |c [Daniel Solaiman, Richard Ashby, Nereus Gunther IV, Jonathan Zerkowski] |
| 520 | 3 | |a We previously discovered that Pseudomonas chlororaphis NRRL B-30761 produces monorhamnolipids (R1Ls) with predominantly 3-hydroxydodecenoyl-3-hydroxydecanoate (C12:1-C10) or 3-hydroxydodecanoyl-3-hydroxydecanoate (C12-C10) as the lipid moiety under static growth conditions only. We have now cloned, sequenced, and analyzed in silico the gene locus of NRRL B-30761 containing the putative coding sequences of rhamnosyltransferase chain A (rhlA Pch , 894bps), rhamnosyltransferase chain B (rhlB Pch , 1272bps), and N-acyl-homoserine lactone-dependent transcriptional regulatory protein (rhlR Pch , 726bps). The putative gene products RhlAPch (297 amino acid residues or a.a.), RhlBPch (423 a.a.), and RhlRPch (241 a.a.) only have between 60 and 65% a.a. identities to their respective closest matched homologs in P. aeruginosa. Polymerase chain reaction (PCR)-based assay did not detect the presence of rhamnosyltransferase C gene (rhlC) in P. chlororaphis, suggesting a genetic basis for the lack of dirhamnose-lipid (R2L) synthesis in this organism. We thus genetically constructed an R2L-synthesizing P. chlororaphis by expressing a rhamnosyltransferase C (rhlC) gene of P. aeruginosa using an expression vector (pBS29-P2-gfp) containing a Pseudomonas syringae promoter. The R2L/R1L ratio is 2.4 in the rhamnolipid (RL) sample isolated from the genetically engineered (GE) P. chlororaphis [pBS29-P2-rhlC], in contrast to undetectable R2L in the GE P. chlororaphis [pBS29-P2-gfp] control cells based on LC-MS analysis. The critical micelle concentrations of the R2L and R1L samples from GE P. chlororaphis [pBS29-P2-rhlC] and the control [pBS29-P2-gfp] cells were ca. 0.1mM, and their minimum surface tensions were ca. 26mN/m with no significant difference. | |
| 540 | |a Springer-Verlag Berlin Heidelberg (outside the USA), 2015 | ||
| 690 | 7 | |a Biosurfactant |2 nationallicence | |
| 690 | 7 | |a Microbial glycolipid |2 nationallicence | |
| 690 | 7 | |a Pseudomonas chlororaphis |2 nationallicence | |
| 690 | 7 | |a Rhamnolipid |2 nationallicence | |
| 690 | 7 | |a Rhamnosyltransferases |2 nationallicence | |
| 690 | 7 | |a Surface tension |2 nationallicence | |
| 700 | 1 | |a Solaiman |D Daniel |u Eastern Regional Research Center, Agricultural Research Service, US Department of Agriculture, 600 E. Mermaid Lane, 19038, Wyndmoor, PA, USA |4 aut | |
| 700 | 1 | |a Ashby |D Richard |u Eastern Regional Research Center, Agricultural Research Service, US Department of Agriculture, 600 E. Mermaid Lane, 19038, Wyndmoor, PA, USA |4 aut | |
| 700 | 1 | |a Gunther IV |D Nereus |u Eastern Regional Research Center, Agricultural Research Service, US Department of Agriculture, 600 E. Mermaid Lane, 19038, Wyndmoor, PA, USA |4 aut | |
| 700 | 1 | |a Zerkowski |D Jonathan |u Eastern Regional Research Center, Agricultural Research Service, US Department of Agriculture, 600 E. Mermaid Lane, 19038, Wyndmoor, PA, USA |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/10(2015-05-01), 4333-4342 |x 0175-7598 |q 99:10<4333 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6433-4 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6433-4 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Solaiman |D Daniel |u Eastern Regional Research Center, Agricultural Research Service, US Department of Agriculture, 600 E. Mermaid Lane, 19038, Wyndmoor, PA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ashby |D Richard |u Eastern Regional Research Center, Agricultural Research Service, US Department of Agriculture, 600 E. Mermaid Lane, 19038, Wyndmoor, PA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gunther IV |D Nereus |u Eastern Regional Research Center, Agricultural Research Service, US Department of Agriculture, 600 E. Mermaid Lane, 19038, Wyndmoor, PA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zerkowski |D Jonathan |u Eastern Regional Research Center, Agricultural Research Service, US Department of Agriculture, 600 E. Mermaid Lane, 19038, Wyndmoor, PA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/10(2015-05-01), 4333-4342 |x 0175-7598 |q 99:10<4333 |1 2015 |2 99 |o 253 | ||