Bacilysin overproduction in Bacillus amyloliquefaciens FZB42 markerless derivative strains FZBREP and FZBSPA enhances antibacterial activity
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6251-0 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6251-0 | ||
| 245 | 0 | 0 | |a Bacilysin overproduction in Bacillus amyloliquefaciens FZB42 markerless derivative strains FZBREP and FZBSPA enhances antibacterial activity |h [Elektronische Daten] |c [Liming Wu, Huijun Wu, Lina Chen, Ling Lin, Rainer Borriss, Xuewen Gao] |
| 520 | 3 | |a Bacillus amyloliquefaciens strains FZBREP and FZBSPA were derived from the wild-type FZB42 by replacement of the native bacilysin operon promoter with constitutive promoters P repB and P spac from plasmids pMK3 and pLOSS, respectively. These strains contained two antibiotic resistance genes, and markerless strains were constructed by deleting the chloramphenicol resistance cassette and promoter region bordered by two lox sites (lox71 and lox66) using Cre recombinase expressed from the temperature-sensitive vector pLOSS-cre. The vector-encoded spectinomycin resistance gene was removed by high temperature (50°C) treatment. RT-PCR and qRT-PCR results indicated that P repB and especially P spac significantly increased expression of the bac operon, and FZBREP and FZBSPA strains produced up to 170.4 and 315.6% more bacilysin than wild type, respectively. Bacilysin overproduction was accompanied by enhancement of the antagonistic activities against Staphylococcus aureus (an indicator of bacilysin) and Clavibacter michiganense subsp. sepedonicum (the causative agent of potato ring rot). Both the size and degree of ring rot-associated necrotic tubers were decreased compared with the wild-type strain, which confirmed the protective effects and biocontrol potential of these genetically engineered strains. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Bacillus amyloliquefaciens |2 nationallicence | |
| 690 | 7 | |a Bacilysin |2 nationallicence | |
| 690 | 7 | |a Promoter replacement |2 nationallicence | |
| 690 | 7 | |a Markerless |2 nationallicence | |
| 690 | 7 | |a Antibiotic overproduction |2 nationallicence | |
| 690 | 7 | |a Antibacterial |2 nationallicence | |
| 700 | 1 | |a Wu |D Liming |u Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, China |4 aut | |
| 700 | 1 | |a Wu |D Huijun |u Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, China |4 aut | |
| 700 | 1 | |a Chen |D Lina |u Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, China |4 aut | |
| 700 | 1 | |a Lin |D Ling |u Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, China |4 aut | |
| 700 | 1 | |a Borriss |D Rainer |u ABiTEP GmbH, Berlin, Germany |4 aut | |
| 700 | 1 | |a Gao |D Xuewen |u Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/10(2015-05-01), 4255-4263 |x 0175-7598 |q 99:10<4255 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6251-0 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6251-0 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wu |D Liming |u Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wu |D Huijun |u Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Chen |D Lina |u Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lin |D Ling |u Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Borriss |D Rainer |u ABiTEP GmbH, Berlin, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gao |D Xuewen |u Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/10(2015-05-01), 4255-4263 |x 0175-7598 |q 99:10<4255 |1 2015 |2 99 |o 253 | ||