caa a22 4500 60550220X CHVBK 20210128100604.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00253-014-6367-2 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6367-2 Correlation of nitric oxide produced by an inducible nitric oxide synthase-like protein with enhanced expression of the phenylpropanoid pathway in Inonotus obliquus cocultured with Phellinus morii [Elektronische Daten] [Yanxia Zhao, Qi Xi, Qian Xu, Meihong He, Jianing Ding, Yucheng Dai, Nancy Keller, Weifa Zheng] Fungal interspecific interactions enhance biosynthesis of phenylpropanoid metabolites (PM), and production of nitric oxide (NO) is known to be involved in this process. However, it remains unknown which signaling pathway(s) or regulator(s) mediate fungal PM biosynthesis. In this study, we cocultured two white-rot fungi, Inonotus obliquus and Phellinus morii, to examine NO production, expression of the genes involved in phenylpropanoid metabolism and accumulation of phenylpropanoid-derived polyphenols by I. obliquus. Coculture of the two fungi caused an enhanced NO biosynthesis followed by increased transcription of the genes encoding phenylalanine ammonia lyase (PAL) and 4-coumarate CoA ligase (4CL), as well as an upregulated biosynthesis of styrylpyrone polyphenols in I. obliquus. Addition of the NO synthase (NOS) selective inhibitor aminoguanidine (AG) inhibited NO production by more than 90% followed by cease in transcription of PAL and 4Cl. Treatment of guanylyl cyclase inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one did not affect NO production but suppressed transcription of PAL and 4CL and reduced accumulation of total phenolic constituents. Genome-wide analysis of I. obliquus revealed two genes encoding a constitutive and an inducible NOS-like protein, respectively (cNOSL and iNOSL). Coculture of the two fungi did not increase the expression of the cNOSL gene but triggered expression of the iNOSL gene. Cloned iNOSL from Escherichia coli shows higher activity in transferring l-arginine to NO, and this activity is lost upon AG addition. Thus, iNOSL is more responsible for NO production in I. obliquus and may act as an important regulator governing PM production during fungal interspecific interactions. Springer-Verlag Berlin Heidelberg, 2015 Inonotus obliquus nationallicence Phellinus morii nationallicence Coculture nationallicence Fungal interspecific interactions nationallicence NOS-like proteins nationallicence Phenylpropanoid metabolism nationallicence Zhao Yanxia Key Laboratory for Biotechnology on Medicinal Plants, Jiangsu Normal University, 221116, Xuzhou, China aut Xi Qi Key Laboratory for Biotechnology on Medicinal Plants, Jiangsu Normal University, 221116, Xuzhou, China aut Xu Qian Key Laboratory for Biotechnology on Medicinal Plants, Jiangsu Normal University, 221116, Xuzhou, China aut He Meihong Key Laboratory for Biotechnology on Medicinal Plants, Jiangsu Normal University, 221116, Xuzhou, China aut Ding Jianing Key Laboratory for Biotechnology on Medicinal Plants, Jiangsu Normal University, 221116, Xuzhou, China aut Dai Yucheng Institute of Microbiology and Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, 100083, Beijing, China aut Keller Nancy Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, 53706, Madison, WI, USA aut Zheng Weifa Key Laboratory for Biotechnology on Medicinal Plants, Jiangsu Normal University, 221116, Xuzhou, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4361-4372 0175-7598 99:10<4361 2015 99 253 https://doi.org/10.1007/s00253-014-6367-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6367-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Yanxia Key Laboratory for Biotechnology on Medicinal Plants, Jiangsu Normal University, 221116, Xuzhou, China aut NATIONALLICENCE 700 1- Xi Qi Key Laboratory for Biotechnology on Medicinal Plants, Jiangsu Normal University, 221116, Xuzhou, China aut NATIONALLICENCE 700 1- Xu Qian Key Laboratory for Biotechnology on Medicinal Plants, Jiangsu Normal University, 221116, Xuzhou, China aut NATIONALLICENCE 700 1- He Meihong Key Laboratory for Biotechnology on Medicinal Plants, Jiangsu Normal University, 221116, Xuzhou, China aut NATIONALLICENCE 700 1- Ding Jianing Key Laboratory for Biotechnology on Medicinal Plants, Jiangsu Normal University, 221116, Xuzhou, China aut NATIONALLICENCE 700 1- Dai Yucheng Institute of Microbiology and Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, 100083, Beijing, China aut NATIONALLICENCE 700 1- Keller Nancy Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, 53706, Madison, WI, USA aut NATIONALLICENCE 700 1- Zheng Weifa Key Laboratory for Biotechnology on Medicinal Plants, Jiangsu Normal University, 221116, Xuzhou, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4361-4372 0175-7598 99:10<4361 2015 99 253