caa a22 4500 605502218 CHVBK 20210128100604.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00253-015-6407-6 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6407-6 Suppression of dust mite allergy by mucosal delivery of a hypoallergenic derivative in a mouse model [Elektronische Daten] [Chunqing Ai, Qiuxiang Zhang, Junrong Ding, Chengcheng Ren, Gang Wang, Xiaoming Liu, Fengwei Tian, Jianxin Zhao, Hao Zhang, Yong Chen, Wei Chen] Allergic asthma caused by house dust mite (HDM) is becoming a public health problem. Specific immunotherapy is considered to be the only curative treatment, but it is always associated with IgE-mediated side effects in the therapy process. A few studies showed that the disruption of allergen IgE epitopes could reduce IgE reactivity and thus reduce allergenic activity. In this study, a hypoallergenic derivative of the major HDM allergen Der p2 was constructed by genetic engineering. This derivative was confirmed to have a considerably reduced IgE reactivity compared with Der p2. For its application in vivo, recombinant Lactococcus lactis (LL-DM) was engineered to deliver the Der p2 derivative to the intestinal mucosal surface. Oral administration of LL-DM significantly alleviated Der p2-induced airway inflammation, as shown by reduced inflammatory infiltration and a reduction in Th2 cytokines in bronchoalveolar lavage. This protective effect was associated with an up-regulation of specific IgG2a and a decrease in IL-4 level in the spleen which may affect specific IgE response. Moreover, the levels of regulatory T cells in the mesenteric lymph nodes and spleen were markedly increased in mice fed with LL-DM, suggesting that LL-DM can inhibit allergic responses via the induction of regulatory T cell. Our results indicate that the Der p2 derivative is a promising therapeutic molecule for specific immunotherapy and recombinant lactic acid bacteria could be developed as a promising treatment or prevention strategy for allergic diseases. Springer-Verlag Berlin Heidelberg, 2015 Hypoallergenic derivative nationallicence Mucosal vaccine nationallicence Lactic acid bacteria nationallicence Allergy nationallicence Ai Chunqing State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, People's Republic of China aut Zhang Qiuxiang State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, People's Republic of China aut Ding Junrong State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, People's Republic of China aut Ren Chengcheng State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, People's Republic of China aut Wang Gang State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, People's Republic of China aut Liu Xiaoming State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, People's Republic of China aut Tian Fengwei State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, People's Republic of China aut Zhao Jianxin State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, People's Republic of China aut Zhang Hao State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, People's Republic of China aut Chen Yong State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, People's Republic of China aut Chen Wei State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4309-4319 0175-7598 99:10<4309 2015 99 253 https://doi.org/10.1007/s00253-015-6407-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6407-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ai Chunqing State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Qiuxiang State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, People's Republic of China aut NATIONALLICENCE 700 1- Ding Junrong State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, People's Republic of China aut NATIONALLICENCE 700 1- Ren Chengcheng State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, People's Republic of China aut NATIONALLICENCE 700 1- Wang Gang State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, People's Republic of China aut NATIONALLICENCE 700 1- Liu Xiaoming State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, People's Republic of China aut NATIONALLICENCE 700 1- Tian Fengwei State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Jianxin State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Hao State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, People's Republic of China aut NATIONALLICENCE 700 1- Chen Yong State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, People's Republic of China aut NATIONALLICENCE 700 1- Chen Wei State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4309-4319 0175-7598 99:10<4309 2015 99 253