caa a22 4500 605502234 CHVBK 20210128100604.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00253-014-6357-4 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6357-4 The relief of microtherm inhibition for p -fluoronitrobenzene mineralization using electrical stimulation at low temperatures [Elektronische Daten] [Xueqin Zhang, Huajun Feng, Yuxiang Liang, Zhiqing Zhao, Yuyang Long, Yuan Fang, Meizhen Wang, Jun Yin, Dongsheng Shen] Low temperature aggravates biological treatment of refractory p-fluoronitrobenzene (p-FNB) because of microtherm inhibition of microbial activity. Considering the potential characterization of energy supply for microbial metabolism and spurring microbial activity by electrical stimulation, a bioelectrochemical system (BES) was established to provide sustaining electrical stimulation for p-FNB mineralization at a low temperature. Electrical stimulation facilitated p-FNB treatment and bioelectrochemical reaction rate constants for the removal and defluorination of p-FNB at 10°C were 0.0931 and 0.0054h−1, which were higher than the sums of the rates found using a biological system and an electrocatalytic system by 62.8 and 64.8%, respectively. At a low temperature, microbial activity in terms of dehydrogenase and ATPase was found to be higher with electrical stimulation, being 121.1 and 100.1% more active than that in the biological system. Moreover, stronger antioxidant ability was observed in the BES, which implied a better cold-resistance and relief of microtherm inhibition by electrical stimulation. Bacterial diversity analysis revealed a significant evolution of microbial community by electrical stimulation, and Clostridia was uniquely enriched. One bacterial sequence close to Pseudomonas became uniquely predominant, which appeared to be crucial for excellent p-FNB treatment performance in the BES at a low temperature. Economic evaluation revealed that the energy required to mineralize an extra mole of p-FNB was found to be 247 times higher by heating the system than by application of electrical stimulation. These results indicated that application of electrical stimulation is extremely promising for treating refractory waste at low temperatures. Springer-Verlag Berlin Heidelberg, 2015 Electrical stimulation nationallicence p -Fluoronitrobenzene nationallicence Low temperature nationallicence Economic evaluation nationallicence Zhang Xueqin School of Environmental Science and Engineering, Zhejiang Gongshang University, 310012, Hangzhou, China aut Feng Huajun School of Environmental Science and Engineering, Zhejiang Gongshang University, 310012, Hangzhou, China aut Liang Yuxiang School of Environmental Science and Engineering, Zhejiang Gongshang University, 310012, Hangzhou, China aut Zhao Zhiqing College of Chemical and Material Engineering, Quzhou University, 324004, Quzhou, China aut Long Yuyang School of Environmental Science and Engineering, Zhejiang Gongshang University, 310012, Hangzhou, China aut Fang Yuan School of Environmental Science and Engineering, Zhejiang Gongshang University, 310012, Hangzhou, China aut Wang Meizhen School of Environmental Science and Engineering, Zhejiang Gongshang University, 310012, Hangzhou, China aut Yin Jun School of Environmental Science and Engineering, Zhejiang Gongshang University, 310012, Hangzhou, China aut Shen Dongsheng School of Environmental Science and Engineering, Zhejiang Gongshang University, 310012, Hangzhou, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4485-4494 0175-7598 99:10<4485 2015 99 253 https://doi.org/10.1007/s00253-014-6357-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6357-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Xueqin School of Environmental Science and Engineering, Zhejiang Gongshang University, 310012, Hangzhou, China aut NATIONALLICENCE 700 1- Feng Huajun School of Environmental Science and Engineering, Zhejiang Gongshang University, 310012, Hangzhou, China aut NATIONALLICENCE 700 1- Liang Yuxiang School of Environmental Science and Engineering, Zhejiang Gongshang University, 310012, Hangzhou, China aut NATIONALLICENCE 700 1- Zhao Zhiqing College of Chemical and Material Engineering, Quzhou University, 324004, Quzhou, China aut NATIONALLICENCE 700 1- Long Yuyang School of Environmental Science and Engineering, Zhejiang Gongshang University, 310012, Hangzhou, China aut NATIONALLICENCE 700 1- Fang Yuan School of Environmental Science and Engineering, Zhejiang Gongshang University, 310012, Hangzhou, China aut NATIONALLICENCE 700 1- Wang Meizhen School of Environmental Science and Engineering, Zhejiang Gongshang University, 310012, Hangzhou, China aut NATIONALLICENCE 700 1- Yin Jun School of Environmental Science and Engineering, Zhejiang Gongshang University, 310012, Hangzhou, China aut NATIONALLICENCE 700 1- Shen Dongsheng School of Environmental Science and Engineering, Zhejiang Gongshang University, 310012, Hangzhou, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4485-4494 0175-7598 99:10<4485 2015 99 253