caa a22 4500 605502242 CHVBK 20210128100604.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00253-015-6509-1 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6509-1 Antimicrobial lubricant formulations containing poly(hydroxybenzene)-trimethoprim conjugates synthesized by tyrosinase [Elektronische Daten] [Idalina Gonçalves, Cláudia Botelho, Ana Teixeira, Ana Abreu, Loïc Hilliou, Carla Silva, Artur Cavaco-Paulo] Poly(hydroxybenzene)-trimethoprim conjugates were prepared using methylparaben as substrate of the oxidative enzyme tyrosinase. MALDI-TOF MS analysis showed that the enzymatic oxidation of methylparaben alone leads to the poly(hydroxybenzene) formation. In the presence of trimethoprim, the methylparaben tyrosinase oxidation leads poly(hydroxybenzene)-trimethoprim conjugates. All of these compounds were incorporated into lubricant hydroxyethyl cellulose/glycerol mixtures. Poly(hydroxybenzene)-trimethoprim conjugates were the most effective phenolic structures against the bacterial growth reducing by 96 and 97% of Escherichia coli and Staphylococcus epidermidis suspensions, respectively (after 24h). A novel enzymatic strategy to produce antimicrobial poly(hydroxybenzene)-antibiotic conjugates is proposed here for a wide range of applications on the biomedical field. Springer-Verlag Berlin Heidelberg, 2015 Methylparaben nationallicence Trimethoprim nationallicence Tyrosinase nationallicence Poly(hydroxybenzene) nationallicence Conjugates nationallicence Antimicrobial nationallicence Gonçalves Idalina CEB - Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut Botelho Cláudia CEB - Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut Teixeira Ana CEB - Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut Abreu Ana CEB - Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut Hilliou Loïc Department of Polymer Engineering, University of Minho, 4800-058, Guimarães, Portugal aut Silva Carla CEB - Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut Cavaco-Paulo Artur CEB - Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4225-4235 0175-7598 99:10<4225 2015 99 253 https://doi.org/10.1007/s00253-015-6509-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6509-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gonçalves Idalina CEB - Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut NATIONALLICENCE 700 1- Botelho Cláudia CEB - Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut NATIONALLICENCE 700 1- Teixeira Ana CEB - Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut NATIONALLICENCE 700 1- Abreu Ana CEB - Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut NATIONALLICENCE 700 1- Hilliou Loïc Department of Polymer Engineering, University of Minho, 4800-058, Guimarães, Portugal aut NATIONALLICENCE 700 1- Silva Carla CEB - Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut NATIONALLICENCE 700 1- Cavaco-Paulo Artur CEB - Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4225-4235 0175-7598 99:10<4225 2015 99 253