caa a22 4500 605502269 CHVBK 20210128100604.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00253-015-6521-5 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6521-5 Simultaneous saccharification and fermentation of cellulose in ionic liquid for efficient production of α-ketoglutaric acid by Yarrowia lipolytica [Elektronische Daten] [Seunghyun Ryu, Nicole Labbé, Cong Trinh] Ionic liquids (ILs) are benign solvents that are highly effective for biomass pretreatment. However, their applications for scale-up biorefinery are limited due to multiple expensive IL recovery and separation steps that are required. To overcome this limitation, it is very critical to develop a compatible enzymatic and microbial biocatalyst system to carry the simultaneous saccharification and fermentation in IL environments (SSF-IL). While enzymatic biocatalysts have been demonstrated to be compatible with various IL environments, it is challenging to develop microbial biocatalysts that can thrive and perform efficient biotransformation under the same conditions (pH and temperature). In this study, we harnessed the robust metabolism of Yarrowia lipolytica as a microbial platform highly compatible with the IL environments such as 1-ethyl-3-methylimidazolium acetate ([EMIM][OAc]). We optimized the enzymatic and microbial biocatalyst system using commercial cellulases and demonstrated the capability of Y. lipolytica to convert cellulose into high-value organics such as α-ketoglutaric acid (KGA) in the SSF-IL process at relatively low temperature 28°C and high pH6.3. We showed that SSF-IL not only enhanced the enzymatic saccharification but also produced KGA up to 92% of the maximum theoretical yield. Springer-Verlag Berlin Heidelberg, 2015 Yarrowia lipolytica nationallicence α-Ketoglutaric acid nationallicence Simultaneous saccharification and fermentation in ionic liquid nationallicence SSF-IL nationallicence 1-Ethyl-3-methylimidazolium acetate nationallicence Ionic liquid nationallicence Ryu Seunghyun Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, USA aut Labbé Nicole Center of Renewable Carbon, University of Tennessee, Knoxville, USA aut Trinh Cong Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, USA aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4237-4244 0175-7598 99:10<4237 2015 99 253 https://doi.org/10.1007/s00253-015-6521-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6521-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ryu Seunghyun Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, USA aut NATIONALLICENCE 700 1- Labbé Nicole Center of Renewable Carbon, University of Tennessee, Knoxville, USA aut NATIONALLICENCE 700 1- Trinh Cong Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4237-4244 0175-7598 99:10<4237 2015 99 253