caa a22 4500 605502277 CHVBK 20210128100605.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00253-015-6378-7 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6378-7 Enzyme multilayer coatings inhibit Pseudomonas aeruginosa biofilm formation on urinary catheters [Elektronische Daten] [Kristina Ivanova, Margarida Fernandes, Ernest Mendoza, Tzanko Tzanov] Bacteria use a signaling mechanism called quorum sensing (QS) to form complex communities of surface-attached cells known as biofilms. This protective mode of growth allows them to resist antibiotic treatment and originates the majority of hospital-acquired infections. Emerging alternatives to control biofilm-associated infections and multidrug resistance development interfere with bacterial QS pathways, exerting less selective pressure on bacterial population. In this study, biologically stable coatings comprising the QS disrupting enzyme acylase were built on silicone urinary catheters using a layer-by-layer technique. This was achieved by the alternate deposition of negatively charged enzyme and positively charged polyethylenimine. The acylase-coated catheters efficiently quenched the QS in the biosensor strain Chromobacterium violaceum CECT 5999, demonstrated by approximately 50% inhibition of violacein production. These enzyme multilayer coatings significantly reduced the Pseudomonas aeruginosa ATCC 10145 biofilm formation under static and dynamic conditions in an in vitro catheterized bladder model. The quorum quenching enzyme coatings did not affect the viability of the human fibroblasts (BJ-5ta) over 7days, corresponding to the extended useful life of urinary catheters. Such enzyme-based approach could be an alternative to the conventional antibiotic treatment for prevention of biofilm-associated urinary tract infections. Springer-Verlag Berlin Heidelberg, 2015 Quorum quenching nationallicence Acylase nationallicence Biofilm inhibition nationallicence Urinary catheters nationallicence Ivanova Kristina Group of Molecular and Industrial Biotechnology, Department of Chemical Engineering, Universitat Politécnica de Catalunya, Rambla Sant Nebridi 22, 08222, Terrassa, Spain aut Fernandes Margarida Group of Molecular and Industrial Biotechnology, Department of Chemical Engineering, Universitat Politécnica de Catalunya, Rambla Sant Nebridi 22, 08222, Terrassa, Spain aut Mendoza Ernest Laboratory of Applied Nanomaterials, Center for Research in NanoEngineering, Universitat Politècnica de Catalunya, c/ Pascual I Vila 15, 08028, Barcelona, Spain aut Tzanov Tzanko Group of Molecular and Industrial Biotechnology, Department of Chemical Engineering, Universitat Politécnica de Catalunya, Rambla Sant Nebridi 22, 08222, Terrassa, Spain aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4373-4385 0175-7598 99:10<4373 2015 99 253 https://doi.org/10.1007/s00253-015-6378-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6378-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ivanova Kristina Group of Molecular and Industrial Biotechnology, Department of Chemical Engineering, Universitat Politécnica de Catalunya, Rambla Sant Nebridi 22, 08222, Terrassa, Spain aut NATIONALLICENCE 700 1- Fernandes Margarida Group of Molecular and Industrial Biotechnology, Department of Chemical Engineering, Universitat Politécnica de Catalunya, Rambla Sant Nebridi 22, 08222, Terrassa, Spain aut NATIONALLICENCE 700 1- Mendoza Ernest Laboratory of Applied Nanomaterials, Center for Research in NanoEngineering, Universitat Politècnica de Catalunya, c/ Pascual I Vila 15, 08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Tzanov Tzanko Group of Molecular and Industrial Biotechnology, Department of Chemical Engineering, Universitat Politécnica de Catalunya, Rambla Sant Nebridi 22, 08222, Terrassa, Spain aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4373-4385 0175-7598 99:10<4373 2015 99 253