Cellulase recycling in biorefineries—is it possible?

Verfasser / Beitragende:
[Daniel Gomes, Ana Rodrigues, Lucília Domingues, Miguel Gama]
Ort, Verlag, Jahr:
2015
Enthalten in:
Applied Microbiology and Biotechnology, 99/10(2015-05-01), 4131-4143
Format:
Artikel (online)
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024 7 0 |a 10.1007/s00253-015-6535-z  |2 doi 
035 |a (NATIONALLICENCE)springer-10.1007/s00253-015-6535-z 
245 0 0 |a Cellulase recycling in biorefineries—is it possible?  |h [Elektronische Daten]  |c [Daniel Gomes, Ana Rodrigues, Lucília Domingues, Miguel Gama] 
520 3 |a On a near future, bio-based economy will assume a key role in our lives. Lignocellulosic materials (e.g., agroforestry residues, industrial/solid wastes) represent a cheaper and environmentally friendly option to fossil fuels. Indeed, following suitable processing, they can be metabolized by different microorganisms to produce a wide range of compounds currently obtained by chemical synthesis. However, due to the recalcitrant nature of these materials, they cannot be directly used by microorganisms, the conversion of polysaccharides into simpler sugars being thus required. This conversion, which is usually undertaken enzymatically, represents a significant part on the final cost of the process. This fact has driven intense efforts on the reduction of the enzyme cost following different strategies. Here, we describe the fundamentals of the enzyme recycling technology, more specifically, cellulase recycling. We focus on the main strategies available for the recovery of both the liquid- and solid-bound enzyme fractions and discuss the relevant operational parameters (e.g., composition, temperature, additives, and pH). Although the efforts from the industry and enzyme suppliers are primarily oriented toward the development of enzyme cocktails able to quickly and effectively process biomass, it seems clear by now that enzyme recycling is technically possible. 
540 |a Springer-Verlag Berlin Heidelberg, 2015 
690 7 |a Biorefineries  |2 nationallicence 
690 7 |a Enzyme recycling  |2 nationallicence 
690 7 |a Cellulase-substrate interactions  |2 nationallicence 
690 7 |a Lignocellulosic bioethanol  |2 nationallicence 
690 7 |a Cellulases cost  |2 nationallicence 
700 1 |a Gomes  |D Daniel  |u CEB-Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal  |4 aut 
700 1 |a Rodrigues  |D Ana  |u CEB-Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal  |4 aut 
700 1 |a Domingues  |D Lucília  |u CEB-Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal  |4 aut 
700 1 |a Gama  |D Miguel  |u CEB-Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal  |4 aut 
773 0 |t Applied Microbiology and Biotechnology  |d Springer Berlin Heidelberg  |g 99/10(2015-05-01), 4131-4143  |x 0175-7598  |q 99:10<4131  |1 2015  |2 99  |o 253 
856 4 0 |u https://doi.org/10.1007/s00253-015-6535-z  |q text/html  |z Onlinezugriff via DOI 
898 |a BK010053  |b XK010053  |c XK010000 
900 7 |a Metadata rights reserved  |b Springer special CC-BY-NC licence  |2 nationallicence 
908 |D 1  |a review-article  |2 jats 
949 |B NATIONALLICENCE  |F NATIONALLICENCE  |b NL-springer 
950 |B NATIONALLICENCE  |P 856  |E 40  |u https://doi.org/10.1007/s00253-015-6535-z  |q text/html  |z Onlinezugriff via DOI 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Gomes  |D Daniel  |u CEB-Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Rodrigues  |D Ana  |u CEB-Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Domingues  |D Lucília  |u CEB-Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Gama  |D Miguel  |u CEB-Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal  |4 aut 
950 |B NATIONALLICENCE  |P 773  |E 0-  |t Applied Microbiology and Biotechnology  |d Springer Berlin Heidelberg  |g 99/10(2015-05-01), 4131-4143  |x 0175-7598  |q 99:10<4131  |1 2015  |2 99  |o 253