caa a22 4500 605502285 CHVBK 20210128100605.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00253-015-6535-z doi (NATIONALLICENCE)springer-10.1007/s00253-015-6535-z Cellulase recycling in biorefineries—is it possible? [Elektronische Daten] [Daniel Gomes, Ana Rodrigues, Lucília Domingues, Miguel Gama] On a near future, bio-based economy will assume a key role in our lives. Lignocellulosic materials (e.g., agroforestry residues, industrial/solid wastes) represent a cheaper and environmentally friendly option to fossil fuels. Indeed, following suitable processing, they can be metabolized by different microorganisms to produce a wide range of compounds currently obtained by chemical synthesis. However, due to the recalcitrant nature of these materials, they cannot be directly used by microorganisms, the conversion of polysaccharides into simpler sugars being thus required. This conversion, which is usually undertaken enzymatically, represents a significant part on the final cost of the process. This fact has driven intense efforts on the reduction of the enzyme cost following different strategies. Here, we describe the fundamentals of the enzyme recycling technology, more specifically, cellulase recycling. We focus on the main strategies available for the recovery of both the liquid- and solid-bound enzyme fractions and discuss the relevant operational parameters (e.g., composition, temperature, additives, and pH). Although the efforts from the industry and enzyme suppliers are primarily oriented toward the development of enzyme cocktails able to quickly and effectively process biomass, it seems clear by now that enzyme recycling is technically possible. Springer-Verlag Berlin Heidelberg, 2015 Biorefineries nationallicence Enzyme recycling nationallicence Cellulase-substrate interactions nationallicence Lignocellulosic bioethanol nationallicence Cellulases cost nationallicence Gomes Daniel CEB-Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal aut Rodrigues Ana CEB-Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal aut Domingues Lucília CEB-Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal aut Gama Miguel CEB-Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4131-4143 0175-7598 99:10<4131 2015 99 253 https://doi.org/10.1007/s00253-015-6535-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6535-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gomes Daniel CEB-Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal aut NATIONALLICENCE 700 1- Rodrigues Ana CEB-Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal aut NATIONALLICENCE 700 1- Domingues Lucília CEB-Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal aut NATIONALLICENCE 700 1- Gama Miguel CEB-Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4131-4143 0175-7598 99:10<4131 2015 99 253