Identification and characterization of a novel locus in Cytophaga hutchinsonii involved in colony spreading and cellulose digestion
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 605502307 | ||
| 003 | CHVBK | ||
| 005 | 20210128100605.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 210128e20150501xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6412-9 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6412-9 | ||
| 245 | 0 | 0 | |a Identification and characterization of a novel locus in Cytophaga hutchinsonii involved in colony spreading and cellulose digestion |h [Elektronische Daten] |c [Hong Zhou, Xia Wang, Tengteng Yang, Weixin Zhang, Guanjun Chen, Weifeng Liu] |
| 520 | 3 | |a Cytophaga hutchinsonii, an aerobic cellulolytic soil bacterium, is capable of degrading crystalline cellulose and gliding over surface rapidly. The involved mechanisms, however, are largely unknown. Here, we used the mariner-based transposon HimarEm1 to screen for C. hutchinsonii mutants deficient in utilizing filter paper as the sole carbon source. A novel gene locus, chu_1719, encoding a hypothetical protein was identified, whose inactivation resulted in a compromised growth of C. hutchinsonii on filter paper. Further analysis revealed that disruption of chu_1719 suppressed colony spreading but had no significant effect on Avicel degradation in liquid medium. Carboxymethylcellulase (CMCase) activity of the mutant membrane proteins was reduced by about 40% as compared with the wild-type strain. Moreover, profiles of cellulose-adsorbed outer membrane proteins were significantly different between the mutant and wild-type (WT) strains. These results suggest that chu_1719 plays an important role in controlling the spreading motility and cellulose utilization probably by affecting the appropriate production of membrane proteins in C. hutchinsonii. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Cytophaga hutchinsonii |2 nationallicence | |
| 690 | 7 | |a Transposon insertion |2 nationallicence | |
| 690 | 7 | |a Colony spreading |2 nationallicence | |
| 690 | 7 | |a Membrane proteins |2 nationallicence | |
| 690 | 7 | |a Cellulose digestion |2 nationallicence | |
| 700 | 1 | |a Zhou |D Hong |u The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, People's Republic of China |4 aut | |
| 700 | 1 | |a Wang |D Xia |u The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, People's Republic of China |4 aut | |
| 700 | 1 | |a Yang |D Tengteng |u The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, People's Republic of China |4 aut | |
| 700 | 1 | |a Zhang |D Weixin |u The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, People's Republic of China |4 aut | |
| 700 | 1 | |a Chen |D Guanjun |u The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, People's Republic of China |4 aut | |
| 700 | 1 | |a Liu |D Weifeng |u The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, People's Republic of China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/10(2015-05-01), 4321-4331 |x 0175-7598 |q 99:10<4321 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6412-9 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6412-9 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhou |D Hong |u The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wang |D Xia |u The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yang |D Tengteng |u The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Weixin |u The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Chen |D Guanjun |u The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Weifeng |u The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/10(2015-05-01), 4321-4331 |x 0175-7598 |q 99:10<4321 |1 2015 |2 99 |o 253 | ||