caa a22 4500 605502307 CHVBK 20210128100605.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00253-015-6412-9 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6412-9 Identification and characterization of a novel locus in Cytophaga hutchinsonii involved in colony spreading and cellulose digestion [Elektronische Daten] [Hong Zhou, Xia Wang, Tengteng Yang, Weixin Zhang, Guanjun Chen, Weifeng Liu] Cytophaga hutchinsonii, an aerobic cellulolytic soil bacterium, is capable of degrading crystalline cellulose and gliding over surface rapidly. The involved mechanisms, however, are largely unknown. Here, we used the mariner-based transposon HimarEm1 to screen for C. hutchinsonii mutants deficient in utilizing filter paper as the sole carbon source. A novel gene locus, chu_1719, encoding a hypothetical protein was identified, whose inactivation resulted in a compromised growth of C. hutchinsonii on filter paper. Further analysis revealed that disruption of chu_1719 suppressed colony spreading but had no significant effect on Avicel degradation in liquid medium. Carboxymethylcellulase (CMCase) activity of the mutant membrane proteins was reduced by about 40% as compared with the wild-type strain. Moreover, profiles of cellulose-adsorbed outer membrane proteins were significantly different between the mutant and wild-type (WT) strains. These results suggest that chu_1719 plays an important role in controlling the spreading motility and cellulose utilization probably by affecting the appropriate production of membrane proteins in C. hutchinsonii. Springer-Verlag Berlin Heidelberg, 2015 Cytophaga hutchinsonii nationallicence Transposon insertion nationallicence Colony spreading nationallicence Membrane proteins nationallicence Cellulose digestion nationallicence Zhou Hong The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, People's Republic of China aut Wang Xia The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, People's Republic of China aut Yang Tengteng The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, People's Republic of China aut Zhang Weixin The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, People's Republic of China aut Chen Guanjun The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, People's Republic of China aut Liu Weifeng The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4321-4331 0175-7598 99:10<4321 2015 99 253 https://doi.org/10.1007/s00253-015-6412-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6412-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Hong The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Wang Xia The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Yang Tengteng The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Weixin The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Chen Guanjun The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Liu Weifeng The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, 250100, Jinan, Shandong, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/10(2015-05-01), 4321-4331 0175-7598 99:10<4321 2015 99 253